نتایج جستجو برای: periplasm
تعداد نتایج: 3548 فیلتر نتایج به سال:
To characterize the cellular stress by n-alkanols with different alkyl chain lengths in Escherichia coli, we investigated how damage cell envelope permeability and whether they enhance promoter activity of response regulator, σE, using variants green fluorescent protein (GFP). By E. coli cells having GFPuv expressing localizing cytoplasm, inner membrane, periplasm, after exposure to n-alkanols,...
T o secrete proteins into their extra-cellular environment, gram-negative bacteria must transport them across both the inner and outer cell membranes , as well as the intervening peptido-glycan-rich periplasm. To date, researchers have identifi ed six different pathways for protein secretion (1). Some of them take secretory proteins directly from the cyto-plasm to the cell exterior, whereas oth...
BACKGROUND Single chain Fvs (scFvs) are widely applied in research, diagnostics and therapeutic settings. Display and selection from combinatorial libraries is the main route to their discovery and many factors influence the success of this process. They exhibit low thermodynamic stability, resulting in low levels of premature cytosolic folding or aggregation which facilitates sec YEG-mediated ...
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