BACKGROUND Polyethyleneimine (PEI), a cationic polymer, is one of the successful and widely used vectors for non-viral gene transfection in vitro. However, its in vivo application was greatly limited due to its high cytotoxicity and short duration of gene expression. To improve its biocompatibility and transfection efficiency, PEI has been modified with PEG, folic acid, and chloroquine in order...

effects of summer savory (satureja hortensis l.) dry powder (ssdp) and essential oil (sseo) on performance, ruminal fermentation and blood metabolites were assessed using 20west azerbaijan native kids (16±1.94 kg initial live weight) in a random block design with 5 treatments (4 replicates per treatment) over a 13-week period. treatments were: i) basal diet without additive (control), ii): basa...

In this study, we enhanced the expression of a plasmid DNA in mesenchymal stem cells (MSC) by the combination of three-dimensional (3D) tissue-engineered scaffold and nonviral gene carrier. To function as an enhanced delivery of plasmid DNA, acetic anhydride was reacted with polyethylenimine (PEI) to acetylate 80% of the primary and 20% of the secondary amines (PEI-Ac(80)). This acetylated PEI ...

Chitosan-graft-polyethylenimine (CHI-g-PEI) copolymer has been used for theimprovement of low transfection efficiency of chitosan. The present study aims to test thepulmonary toxicity and efficiency of CHI-g-PEI as an aerosol gene carrier. Mice were exposedto aerosol containing green-fluorescent protein (GFP)-polyethylenimine (PEI) or GFP-CHIg-PEI complexes for 30 min during the development of ...

This study evaluates the effects of different levels of guanidinoacetic acid (GAA) supplement on growth performance and gut morphology in broilers (Ross 308 strain) raised at high altitude (2100 m). A total of 300 one-day-old male broiler chicks (Ross 308 strain) were used in a completely randomized design with five treatments and four replicate pens of 15 birds in each. Five dietary treatments...

Polyethyleneimine (PEI) is an organic polycation used extensively as a gene and DNA vaccine delivery reagent. Although the DNA targeting activity of PEI is well documented, its immune activating activity is not. We recently reported that PEI has robust mucosal adjuvanticity when administered intranasally with glycoprotein antigens. Here, we show that PEI has strong immune activating activity af...

Graphene as a star in materials research has been attracting tremendous attentions in the past few years in various fields including biomedicine. In this work, for the first time we successfully use graphene as a non-toxic nano-vehicle for efficient gene transfection. Graphene oxide (GO) is bound with cationic polymers, polyethyleneimine (PEI) with two different molecular weights at 1.2 kDa and...

188Re (Rhenium) is easily obtained from an in-house 188W/188Re generator that is similar to the current 99Mo/99mTc generator, making it very convenient for clinical use. This characteristic makes this radionuclide a promising candidate as a therapeutic agent. Polyethylenimine (PEI) is a cationic polymer and has been used as a gene delivery vector. Positively charged materials interact with cell...

Sorghum stemborer species remains key yield limiting factor for sorghum production in the Nigerian semi-arid region. When they can afford, farmers rely almost solely on synthetic insecticides for control. Owing to the misuse and environmental and health challenges attributed to the use of synthetic insecticides, a two-year field study was carried out at the University of Maiduguri Research Farm...