aqueous two-phase systems (atps) have  emerged as a powerful extraction method for the downstream processing of bio-molecules. the aim of this work was to investigate the possibility of utilizing atps for the separation of recombinant bacillus sphaericus phenylalanine dehydrogenase (phedh). polyethylene glycol (peg) and ammonium sulfate systems were selected for our experiment. the effect of di...

Polyethylene glycol (PEG)-based hydrogels, with variable stiffness, are widely used in tissue engineering to investigate substrate stiffness effects on cell properties. Transcriptome analysis is a critical method for understanding cell physiology. However, significant RNA degradation was observed during the process of isolating and purifying RNA from cells encapsulated in the PEG hydrogel, ther...

To assess the risks from viral contamination of drinking-water supplies, there is a clear need for methods to directly detect viral pathogens. In this study, we developed a broad-spectrum immunocapture method for concentration and purification of enteric viruses. The method involved indirect antibody capture (AbCap) of intact viruses followed by release of virion genomic RNA and reverse transcr...

We describe a modification of an existing tip-recording technique for electrophysiologically investigating short, peg-like sensory sensilla. On the mid-ventral surface of all scorpions are two appendages called pectines, which have dense fields of mechano- and chemosensory peg sensilla. One method for assessing chemoresponsiveness of these sensilla uses a tungsten electrode for extracellularly ...

BACKGROUND AND AIMS Peristomal wound infections are common complications of percutaneous endoscopic gastrostomy (PEG). The Funada-style gastropexy device has two parallel needles with a wire loop and suture thread, and was developed about 20 years ago in Japan. This kit has allowed us to perform dual gastropexy very easily; PEG with gastropexy has become a very popular technique in Japan. The p...

The present study mainly compared the effect of docetaxel-albumin nanoparticles (DANPs) and docetaxel-loaded PEG-albumin nanoparticles (PEG-DANPs) against non-small cell lung cancer (NSCLC). We made systematic assessments on these three drugs against NSCLC both in vitro and in vivo. With the purpose of eliminating side-effects of the commercial formulation (Tween-80) and prolonging the blood ci...

An 80-year-old woman with a nasogastric tube in situ for cerebral infarction sequelae was referred for percutaneous endoscopic gastrostomy (PEG). The abdominal computed tomography scan on admission showed that the transverse colon was interposed between the anterior abdominal wall and the stomach (●" Fig.1). The usual PEG placement posed a high riskof piercing the transverse colon. A fluoroscop...

The gut sac is a long-standing, widely used in vitro preparation for studying solute and water transport, and calculation of these fluxes requires an accurate assessment of volume. This is commonly determined gravimetrically by measuring the change in mass over time. While convenient this likely under-estimates actual net water flux (Jv) due to tissue edema. We evaluated whether the popular in ...