Changes in the composition and structure of cell walls and extracellular polysaccharides (ECP) were studied during the growth of suspension-cultured Arabidopsis thaliana microcalli. Three growth phases, namely the cell division phase, the cell expansion phase, and the stationary phase, were distinguished and associated with a decreasing cell cluster adhesion strength. Degradation of the homogal...

Background: Osteoarthritis (OA) is a progressive, age-associated disease that is characterized with cartilage destruction, subchondral bone remodeling and inflammation of the synovial membrane. Considering the complications of common treatments of OA, including non-steroidal anti-inflammatory drug (NSAIDs) and corticosteroids, investigating new treatments for this disorder is crucial. Recently,...

The changes of microorganisms and pectic substances of partially processed carrots (PPC) were determined with respect to cutting direction. The increase of microbial counts occurred in all tissues and both lengthwise cut sections (LCS)and crosswise cut sections (CCS). The microbial count in the xylem of LCS increased at a greater rate than those of phloem at 20•Ž. The counts in both tissues of ...

introduction: in the present study, we hypothesized that a novel approach to promote vascularization would be to create injectable three dimensional (3-d) scaffolds within growth factor that enhance the sustained release of growth factor and induce the angiogenesis. material and methods: we demonstrate that a 3-d scaffold can be formed by mixing of peptide-amphiphile (pa) aqueous solution with ...

Phytic acid (PA) has been recognized as a potent antioxidant and inhibitor of iron-catalyzed hydroxyl radical formation under in vitro and in vivo conditions. Therefore, the aim of the present study was to investigate, with the use of HPLC/MS/MS, whether PA is capable of inhibiting linoleic acid autoxidation and Fe(II)/ascorbate-induced peroxidation, as well as Fe(II)/ascorbate-induced lipid pe...

Indoleacetic acid (IAA) and/or inhibitors of DNA, RNA or protein synthesis were added to the apex of decapitated seedlings of Pisum sativum L. var. Alaska. At various times up to 4 days, enzymic protein was extraoted from a segment of epicotyl immediately below the apex and assayed for its ability to hydrolyse polysaccharides or their derivatives. With the exception of am;ylase, the total amoun...

The partial purification and characterization of cell wall polysaccharides isolated from suspension-cultured Douglas fir (Pseudotsuga menziesii) cells are described. Extraction of isolated cell walls with 1.0 m LiCl solubilized pectic polysaccharides with glycosyl-linkage compositions similar to those of rhamnogalacturonans I and II, pectic polysaccharides isolated from walls of suspension-cult...

The aim of this work was to investigate heat-induced changes in cell wall polysaccharides of carrot in relation to texture. Discs of fresh carrot (Daucus carota cv Amstrong) tissue were subjected to cooking (100¡C, 20 min), with or without a pre-cooking treatment (50¡C, 30 min). Alcohol-insoluble residues were prepared from the tissues and were extracted sequentially with water, NaCl, CDTA, and...

Clostridium stercorarium F-9 pectate lyase Pel9A is a modular enzyme composed of two hypothetical family-9 catalytic modules of the polysaccharide lyases, CM9-1 and CM9-2, in order from the N terminus. In this study, we constructed and characterized CM9-1 and CM9-2 polypeptides as rCM9-1 and rCM9-2 respectively. Both of them, like the full-length Pel9A, required the Ca2+ ion for their enzyme ac...