Heosemys grandis , a species of Asian water turtle, that has wide range applications in the food and pharmaceutical industries. Since some processed products cannot be identified exclusively by morphological microscopic identification, reliable quick approach to guarantee authenticity is critical. Thus, we fostered an effective stable molecular identification system identify based on DNA mini-b...

Early and precise pathogen identification corresponding disease management are primary concerns in aquaculture. Here, we attempted at diagnostic methods that can simultaneously identify multiple pathogens, where many samples, several concurrent infections to be handled. Hence, a multiplex PCR assay targeting five major aquaculture viz. Vibrio parahaemolyticus, anguillarum, alginolyticus, vulnif...

BACKGROUND Nucleic acid isolation, the most technically demanding and laborious procedure performed in molecular diagnostics, harbors the potential for improvements in automation. A recent development is the use of magnetic beads covered with nucleic acid-binding matrices. We adapted this technology with a broad-range 23S rRNA real-time reverse transcription (RT)-PCR assay for fast and sensitiv...

Background: Spermatogenesis associated 19 (Spata19) was introduced as a testis-specific gene that probably involved in spermatogenesis cell apoptosis. Therefore, this study aimed to investigate the effect of Spata19 inactivation on sperm count. Materials and Methods: We generated global knockout mice by CRISPR/Cas9 nickase technology. Disability validated three levels DNA, RNA, protein using PC...