We describe a simple method for detection of Plasmodium vivax and Plasmodium falciparum infection in anophelines using a triplex TaqMan real-time polymerase chain reaction (PCR) assay (18S rRNA). We tested the assay on Anopheles darlingi and Anopheles stephensi colony mosquitoes fed with Plasmodium-infected blood meals and in duplicate on field collected An. darlingi. We compared the real-time ...

BACKGROUND Ovine footrot is a contagious disease with worldwide occurrence in sheep. The main causative agent is the fastidious bacterium Dichelobacter nodosus. In Scandinavia, footrot was first diagnosed in Sweden in 2004 and later also in Norway and Denmark. Clinical examination of sheep feet is fundamental to diagnosis of footrot, but D. nodosus should also be detected to confirm the diagnos...

A high-throughput multiplexed assay was developed for the differential laboratory detection of foot-and-mouth disease virus (FMDV) from viruses that cause clinically similar diseases of livestock. This assay simultaneously screens for five RNA and two DNA viruses by using multiplexed reverse transcription-PCR (mRT-PCR) amplification coupled with a microsphere hybridization array and flow-cytome...

Methods such as real time (RT)-PCR have not been developed for the rapid detection and diagnosis of Dermatophilus (D.) congolensis infection. In the present study, a D. congolensis-specific SYBR Green RT-PCR assay was evaluated. The detection limit of the RT-PCR assay was 1 pg of DNA per PCR reaction. No cross-reaction with nucleic acids extracted from Pseudomonas aeruginosa, Mycobacterium tube...

Thesis Abstract Several cytokines are known to be involved in the pathogenesis of non-Hodgkin Lymphomas (NHL). Given importance understanding genetic predisposition NHL, this work was designed study impact IL-10 (-1082 G/A; rs1800896 and -819 C/T; rs1800871), IL-6 (-174 G/C; rs1800795) CD38 (184C/G; rs6449182) gene polymorphisms on susceptibility Egyptians NHL. Both loci IL10 were genotyped usi...

Objective: To perform comparative diagnostic study with rotavirus infected stool samples for efficacy ofindigenously prepared molecular transport medium (IMTM).Study Design: It was an experimental study.Place and Duration of Study: The conducted at Department Microbiology University Karachi,Karachi Pakistan from June to July 2021.Materials Methods: current based on hospital surveillance rotavir...

Aim A spesifi c and rapid diagnosis such as RT-PCR asay is the most needed to minimize transmission of HIV-1 infection. Therefore, in this study we developed the RT-PCR assay that was spesifi c against the gag gene of HIV-1. Methods The developed RT-PCR assay was evaluated against 46 specimens that were obtained from voluntary counseling and testing for HIV (VCT) in Rumah Sakit Umum Pemerintah ...

  Avian Influenza (AI) is a viral respiratory disease of domestic and wild birds. In the diagnostic laboratory, it is essential to have methods for rapid detection of avian respiratory viruses. Cloacal swabs collected from chickens experimentally infected with H9 subtype AI virus, used in a reverse transcription-polymerase chain reaction (RT-PCR) assay for detection of AI. In infected animals, ...

in this study 130 bulk tank milk samples which were delivered to the pegah pasturisation factory inmashhad were collected randomly during the summer months. samples were firstly enriched in modifiedtrypticase soy broth containing novobiocin, followed by plating onto sorbitol macconkey agar supplementedwith cefixime and potassium tellurite for isolation of escherichia coli o157:h7. consequently ...

causes human intestinal cyclosporiasis. It is more common in the immunocompromised patients and mainly seen people living with HIV/AIDS (PLHA), post-renal transplant (PRT) children (IC). Diagnostic microscopy for oocysts of parasite less sensitive, requiring examination multiple stool samples. Here we developed a new single run polymerase chain reaction (PCR) assay detection it was used to know...