Centrosomes consist of two centrioles surrounded by an amorphous pericentriolar matrix (PCM), but it is unknown how centrioles and PCM are connected. We show that the centrioles in Drosophila embryos that lack the centrosomal protein Centrosomin (Cnn) can recruit PCM components but cannot maintain a proper attachment to the PCM. As a result, the centrioles "rocket" around in the embryo and ofte...

The centrosome influences the shape, orientation and activity of the microtubule cytoskeleton. The pericentriolar material (PCM), determines this functionality by providing a dynamic platform for nucleating microtubules and acts as a nexus for molecular signaling. Although great strides have been made in understanding PCM activity, its diffraction-limited size and amorphous appearance on electr...

The sensitivity of bacteria to the bactericidal activity of serum depends on the structure and organization of the bacterial outer membrane. Sialic acid has been found in the O-specific region of bacterial lipopolysaccharide (LPS) and it plays an essential role in protecting Gram-negative bacteria against the bactericidal activity of human and animal serum. The susceptibility of Gram-negative b...

A new simple and sensitive ratio-spectra first derivative spectrophotometric method was developed for the simultaneous determination of tapentadol hydrochloride (TPH) and paracetamol (PCM) in binary mixtures. The method depends on the use of the first derivative of the ratio-spectra obtained by dividing the absorption spectrum of binary mixture by a standard spectrum of one of the compounds. TP...

Experiments have been performed on the thermal behavior of a collector–storage wall system using PCM (phase change material). PCM slabs were attached on the gap-side wall surface to increase the heat storage. The test was carried out for a whole day with charging period of 6.5 h and discharging period of 17.5 h, respectively. Wall and air temperatures as well as air velocity in the gap were mea...

This study was conducted to apply a phase change material (PCM) to the inner skin part of a double skin facade to positively utilize natural solar heat so that a double skin facade may not only counteract external environmental changes but also positively introduce external natural solar energy, highlighting the advantages of a double skin facade. However, PCM is currently used by impregnating ...

Centrosomes comprise a pair of centrioles surrounded by a matrix of pericentriolar material (PCM). In vertebrate cells, Pericentrin plays an important part in mitotic PCM assembly, but the Drosophila Pericentrin-like protein (PLP) appears to have a more minor role in mitotic fly cells. Here we investigate the function of PLP during the rapid mitotic cycles of the early Drosophila embryo. Unexpe...

IgM plasma cell myeloma (PCM) is a rare entity, and CD19 positivity is found in only 1-4% of PCM. Here we report a unique case of IgM PCM, in which the plasma cells are positive for CD19. Clinically, the patient presented with hyperviscosity syndrome, mimicking the clinical manifestation of Waldenstrom's macroglobulinemia. In addition, the IgM para-protein from the patient behaved like cryoglob...

Phase-change materials (PCM) can be used to reduce thermal stress and improve thermal comfort for workers wearing protective clothing. The aim of this study was to investigate the effect of PCM in protective clothing used in simulated work situations. We hypothesized that it would be possible to optimize cooling performance with a design that focuses on careful positioning of PCM, minimizing to...

We present the theory and implementation for computing the (free) energy and its analytical gradients with the Brueckner doubles (BD) coupled cluster method in solution, in combination with the polarizable continuum model of solvation (PCM). The complete model, called PTED, and an efficient approximation, called PTE, are introduced and tested with numerical examples. Implementation details are ...