Department of Medicine, University of Cambridge, Cambridge, UK; Department of Veterinary Medicine, University of Cambridge, Cambridge, UK; Clinical Microbiology and Public Health Laboratory, Public Health England, Cambridge, UK; Royal Veterinary College, Hawkshead Campus, University of London, London, UK; Antimicrobial Resistance and Healthcare Associated Infections Reference Unit, Public Healt...

BACKGROUND & OBJECTIVE Conventional methods to detect methicillin resistance in Staphylococcus aureus are inadequate as expression of resistance is subject to environmental and conditional expression of PBP2a antigen. The objective of the present study was to determine methicillin resistance in S. aureus by conventional susceptibility (oxacillin disc diffusion and oxacillin MIC) and molecular m...

coding regions of the transpeptidase domain of the pbp1a, pbp2b and pbp2x genes of the two GBS isolates were amplified and sequenced according to previously outlined methods. 4 Amino acid sequences were deduced and analysed using the ClustalW alignment tool included in the Lasergene software (DNAstar, Madison, WI, USA). Nucleotide and deduced amino acid sequences were compared with those of the...

Strains of methicillin-resistant Staphylococcus aureus (MRSA) have become the most important causative agents of hospital-acquired diseases worldwide. The genetic determinant of resistance, mecA, is not a gene native to S. aureus but was acquired from an extraspecies source by an unknown mechanism. We recently identified a close homologue of this gene in isolates of Staphylococcus sciuri, a tax...

Staphylococcus aureus strains that are mecA and PBP2a positive but phenotypically susceptible to oxacillin becoming more abundant, according research from all around the world. The susceptibility of (OS-MRSA) contributes consequent treatment-failure due misidentification by conventional tests. Therefore, objective current study was ascertain prevalence OSMRSA in a tertiary care facility located...