Paralysis of skeletal muscles, which can lead to paralysis of respiratory muscles and death, is one of the most toxic effects of organophosphates, and oximes are almost the only known antidotes that can reverse or prevent such toxic effects. In the present research work, possible reversal or preventive effect of different concentrations of the relatively new oxime (HI-6) on changes induced by p...

R,R'-disubstituted sulfoximines were phosphorylated with O,O-diethylchloro phosphate and phosphorothionate to obtain new organophosphorus compounds. After purification they were characterized by GC-MS and (1)H-NMR. The toxicity of the synthesized O,O-diethyl N-(R,R'-disubstituted sulfoximine) phosphoro-amidothionates was assayed on Musca domestica. It was found that the methyl phenyl derivative...

Magnetic Fe(3)O(4) particle aggregates were prepared by cross-linking Fe(3)O(4) nanoparticles bearing surface carbonyl groups with poly-L-lysine. Upon further coupling with antiparaoxon methyl polyclonal antibody, the resultant particle aggregate-based probes were used in a lateral flow immunochromatographic assay (LFIA) of pesticide residue of paraoxon methyl. The results were compared with th...

Excess activation of muscle nicotinic acetylcholine receptors due to genetic mutations, as seen in slow channel congenital myasthenic syndrome, or acetylcholinesterase (AChE) inhibition results in muscle cell degeneration. Our recent work showed that nitric oxide synthase (NOS) inhibitors prevent nicotine-induced muscle cell death in culture. In the present study, we examined the effects of NOS...

The qualitative morphologic study of the effects of organophosphate agents on development of neural cells in culture is a promising approach as a primitive step for further physiologic, electro physiologic and molecular investigation. Parathion is an OP (Organophosphate) used as an agricultural insecticide. Improper handling of this agent causes poisoning characterized by convulsive seizures, w...

One of the most toxic effects of organophosphate (OP) poisoning has been the paralysis of skeletal muscles that can lead to paralysis of respiratory muscles and death. However, oximes are the only antidotes available to reverse or prevent such toxic effects of OP insecticides and nerve chemical warfare agents. In the present research work, the effect of different concentrations of paraoxon (as ...

Paralysis of skeletal muscles, which can lead to paralysis of respiratory muscles and death, is one of the most toxic effects of organophosphates, and oximes are almost the only known antidotes that can reverse or prevent such toxic effects. In the present research work, possible reversal or preventive effect of different concentrations of the relatively new oxime (HI-6) on changes induced by p...

The qualitative morphologic study of the effects of organophosphate agents on development of neural cells in culture is a promising approach as a primitive step for further physiologic, electro physiologic and molecular investigation. Parathion is an OP (Organophosphate) used as an agricultural insecticide. Improper handling of this agent causes poisoning characterized by convulsive seizures, w...

The differentiation of paraoxonase (an A-type esterase in serum) from related enzymes has been complicated by overlapping substrate specificities and by the complex nature of these enzymes. Rabbit-serum paraoxonase has been differentiated from hog-kidney diisopropyl phosphorofluoridatase by Mounter (1954). He confirmed the observation of Aldridge (1953b) that one enzyme in rabbit serum hydrolys...

II The objective of this work was to develop new biosensors for multianalyte detection. Target analytes were cholinesterase inhibiting insecticides and chlorinated aromatic hydrocarbons. The task was fulfilled by a combination of biological receptors bearing different selectivities for the desired analytes and data evaluation by feed-forward artificial neural networks (ANN). The basic sensor de...