OBJECTIVE To assess the frequency of phenotypic expression of inducible resistance of clindamycin due to expression of erm genes, in clinical isolates of Staphylococcus aureus (S. aureus), by double disk diffusion test (D-test). METHOD This was a cross sectional study conducted in the clinical laboratory of Aga Khan University Hospital, Karachi. A total of 2432, non duplicate clinical isolate...

Diabetic retinopathy has been revealed as the most common cause of blindness among people of working age. The colour fundus images have been the easiest way to analyse the eye fundus and prevent the progression of this ocular disease. An important prerequisite for automation is the segmentation of the main anatomical features in the image, particularly the optic disc. Ant Colony Optimization (A...

Staphylococcus epidermidis is the most important member of coagulase negative staphylococci responsible for community and hospital acquired infections. Most clinical isolates of S. epidermidis are resistant to methicillin making these infections difficult to treat. In this study, correlation of methicillin resistance phenotype was compared with methicillin resistance (mecA) gene carriage in 55 ...

K.E.M. Hospital, Parel, Mumbai-400012. Abstract Due to cost and complexity, reference broth microdilution antifungal susceptibility method is not feasible in resource constraint setting. Therefore here is a need to develop a cost effective method which would help in simultaneous rapid identification of Candida species and performance of disc diffusion susceptibility testing. Comparison of resul...

A reference method for the antimicrobial susceptibility testing of common fungal pathogens such as dermatophytes, is currently lacking. In this study, we report the successful adaptation of solid agar-based spot culture growth inhibition assay (SPOTi) for dermatophytes, currently being used as a gold-standard in the anti-tubercular drug discovery field. The fungal-SPOTi assay correlated with th...

Antibiotic sensitivities for Linezolid of 20 strains that reported as Brucella melitensis in blood cultures between 2014 and 2016 were evaluated by disc diffusion and E-test methods. According to the disc diffusion method results, 20 Brucella melitensis strains had ≥ 25 mm of antibiotic sensitivity zones. Etest measurements revealed that MIC values were between 1.5 and 6 μg. Linezolid can be an...