Cln3 cyclin of the budding yeast Saccharomyces cerevisiae is a key regulator of Start, a cell cycle event in G1 phase at which cells become committed to division. The time of Start is sensitive to Cln3 levels, which in turn depend on the balance between synthesis and rapid degradation. Here we report that the breakdown of Cln3 is ubiquitin dependent and involves the ubiquitin-conjugating enzyme...

Survivin is a member of the inhibitor of apoptosis gene family that has been implicated in both apoptosis inhibition and regulation of mitosis. However, the subcellular distribution of survivin has been controversial and variously described as a microtubule-associated protein or chromosomal passenger protein. Here, we show that antibodies directed to the survivin sequence Ala(3)-Ile(19) exclusi...

This paper exploits a large-scale auction experiment conducted by two Chinese government treasury security issuers—the Development Bank and the Export-Import Bank—to investigate whether securities should be sold through uniform price or discriminatory mechanisms. Based on outcomes of more than 300 issued an alternating auction-rule market experiment, we find that yield rates formats are not sta...

PURPOSE We recently demonstrated the strong anticancer efficacy of silibinin,an active constituent of a widely consumed dietary supplement milk thistle extract, against human prostate cancer cells in culture and nude mice xenografts. We also observed that pharmacologically achievable concentrations of silibinin in animal studies were in the range of 25-100 microM, depending on the dose regimen,...

Currently, it is believed that toad oocyte maturation is dependent on the physiological conditions of winter hibernation. Previous antibody-blocking experiments have demonstrated that toad ubiquitin carboxyl-terminal hydrolase L1 (tUCHL1) is necessary for germinal vesicle breakdown during toad oocyte maturation. In this paper, we first supply evidence that tUCHL1 is highly evolutionarily conser...

that this phosphorylation event may contribute to the inhibition of DNA replication activity (12, 13). These studies, in conjunction with the observation that cell lines derived from AT patients lack the irradiation-induced S-phase arrest (i.e., exhibit RDS; Refs. 22 and 23) and are deficient in IR-induced RPA phosphorylation (13, 14), sug gested that RPA phosphorylation may play a functional r...