Nematophagous fungi Pochonia chlamydosporia and P. rubescens colonize endophytically barley roots. During nematode infection, serine proteases are secreted. We have investigated whether such proteases are also produced during root colonization. Polyclonal antibodies against serine protease P32 of P. rubescens cross-reacted with a related protease (VCP1) of P. chlamydosporia, but not with barley...

increases over controls were found when root segments were incubated in phosphorus solutions containing 10-3 A D)NP ancd at lowx temlperatture. The increased Pi in Al-roots did niot result in an increase in the total amounit of l)hosphorus incorporated into phosphorylated compounds. Aluminum treatnment miiarkedly decreased the incorporation of P32 into sigar phosl)hates but increased the pool s...

The region of the resistance plasmid Rl which is transferred first during conjugation (leading region) has been sequenced. It is shown together with the oriT region of this plasmid (1) in the figure. The putative strand-specific cleavage site selected in analogy to the site which was experimentally determined for the F plasmid (2), is designated by the word 'nick . Two open reading frames of op...

Neither the P32-phosphate nor the S35-sulfate were metabolized by intact seeds until after radicle protrusion (15 to 18 hours after beginning of imbibition). Punctured seeds, however, metabolized the radioactive phosphate or sulfate within 3 hours after the beginning of imbibition. We concluded that the coats surrounding the embryo are impermeable to phosphate and sulfate ions. Most of the orga...

Many workers have over many years tried to find a reliable method of detecting damage to the blood supply of the femoral head soon after fracture of the neck of the femur. Up to now the most promising method has been that in which a radioactive isotope is given intravenously and the amount of it appearing in the femoral head and trochanter is measured. In 1950 Tucker published a preliminary rep...

By SHIRLEY EBBE,#{176} MARIO BALDINI AND JANET DONOVAN T HE ABILITY of platelets to survive in the circulation appears to be their most labile property. Platelets may function normally in blood coagulation in the presence of damage severe enough to prevent their circulation when transfused to compatible recipients.1 After storage for 2 weeks, platelets still exhibit clot retraction,2 one of the...

By RICHARD I. WALKER, J. C. HERION AND J. G. PALMER C URRENT EVIDENCE indicates that isotopic labeling of deoxyribonucleic acid ( DNA ) results from synthesis during mitotic interphas&5 and that acquired label is retained until cell death and degradation occur.”5 2 The validity of using inorganic radiophosphorus ( p:oi ) as a leukocyte DNA label rests on these findings. Leukocyte kinetics have ...

E BULK OF DATA initially accumulated regarding the lymphocyte tended to indicate that its life span was relatively short. Thus, Minot and Isaacs,’ in 1925, transfused blood from a patient with chronic lymphocytic leukemia into a patient with lymphosarcoma. The recipient’s blood showed an elevated lymphocyte count for 35 minutes before returning to normal. Yoffey,2 in 1935, showed that in the do...