Protein Kinase (casein kinase 2, CK2) is a serine-threonine kinase that is frequently dysregulated in many human tumors. Therefore we hypothesized that peptides capable of binding to the CK2 acidic domain may exhibit potential anticancer properties. By screening a random cyclic peptide phage display library, we have identified a novel peptide, P15, that abrogated CK2 phosphorylation by blocking...

The nucleoporin gene NUP98 was found fused to the HOXA9, HOXD13, or DDX10 genes in human acute myelogenous leukemia (AML) with chromosome translocations t(7;11)(p15;p15), t(2;11)(q35;p15), or inv(11)(p15;q22), respectively. We report here the fusion between the NUP98 gene and another homeobox gene PMX1 in a case of human AML with a t(1;11)(q23;p15) translocation. The chimeric NUP98-PMX1 transcr...

Frequent deletion of chromosome 9p2l in many cancers has suggested the presence of tumor suppressor genes in this region. Two genes mapping to 9p2l,pl5 andpl6, encode inhibitors for cyclin-dependent kinases 4 and 6.Werecently foundthatinT-cell acutelymphoblastic leukemia (T-ALL), both thepl5 and p16 genes are deleted at a high frequency, with p16 gene deletion occurring slightly more frequently...

Adult hamsters exposed to short photoperiods show a marked atrophy of their internal reproductive organs, including a reduction in size, though not number of Leydig cells. Transforming growth factor-β1 (TGF-β1) is involved in the regulation of growth and proliferation of different cell types. The aim of the present study was to examine the influence of photoperiod on the protein and gene expres...

PURPOSE The tumor suppressor gene Smad4/DPC4, a key transcription factorin transforming growth factor beta (TGF-beta) signaling cascades,is inactivated in 50% of pancreatic adenocarcinomas. We seek to determine the role of Smad4/DPC4 in the suppression of tumor cell growth and in the regulation of TGF-beta-mediated expression of cell-cycle regulatory genes p15(ink4b) and p21(waf1). EXPERIMENT...

Purpose: The tumor suppressor gene Smad4/DPC4, a key transcription factor in transforming growth factor (TGF) signaling cascades, is inactivated in 50% of pancreatic adenocarcinomas. We seek to determine the role of Smad4/DPC4 in the suppression of tumor cell growth and in the regulation of TGF-mediated expression of cell-cycle regulatory genes p15 and p21. Experimental Design: Smad4/DPC4 is ov...

Glioblastomas are the most frequent and malignant brain tumors in adults. Surgical cure is virtually impossible and despite of radiation and chemotherapy the clinical course is very poor. Epigenetic silencing of MGMT has been associated with a better response to temozolomide-chemotherapy. We previously showed that temozolomide increases the median survival time of patients with tumors harbourin...

BACKGROUND Hypermethylation within the promoters of selected genes is an epigenetic pathway that appears to be especially common in all types of human haematopoeitic neoplasms. It is usually associated with inactivation of the involved genes, and can be reversed using demethylating agents. The aim of this study is to evaluate the frequency of p15 and E-cadherin promoter methylation in Egyptian ...

We evaluated the influence of heme oxygenase-1 (HO-1) gene inhibition in myelodysplastic syndrome (MDS) cell line SKM-1 on enhancement of the demethylating effects of decitabine on p15, and explored the possible mechanism. DNMT1 gene expression in SKM-1 cells was silenced by being transfected by a constructed siRNA with liposomes. The proliferation inhibition rates after drug treatment were det...