Objectives: I. Describe the structure of the Mitochondrion. A. Compare the structure of the outer membrane to the structure of the inner membrane. B. What metabolic pathways are localized to the mitochondrion? 1. Where in the mitochondrion are they located? II. What is(are) the overall chemical reaction(s) of electron transport and oxidative phosphorylation? III. Describe the overall makeup of ...

Adipogenesis is accompanied by differentiation of adipose tissuederived stem cells to adipocytes. As part of this differentiation, biogenesis of the oxidative phosphorylation system occurs. Many chemical compounds used in medicine, agriculture or other human activities affect oxidative phosphorylation function. Therefore, these xenobiotics could alter adipogenesis. We have analyzed the effects ...

Experiments are reported showing that in net oxidative phosphorylation by submitochondrial particles ADP furnishes the terminal bridge oxygen in the ATP formed. In contrast to ADP, AMP does not suffice to activate a inorganic orthophosphate -F? HOH exchange by submitochondrial particles. In sensitive initial labeling experiments during net oxidative phosphorylation, “Pi appears initially in ATP...

1. The effects of salicylate on oxidative phosphorylation and respiration were investigated in liver and brain mitochondria, and in sonically prepared mitochondrial fragments. 2. Salicylate was shown to uncouple oxidative phosphorylation in mitochondrial fragments, as well as in intact mitochondria. The effects of salicylate on mitochondria and oxidative phosphorylation resemble those produced ...

The role of cardiolipin in mitochondrial energy transformation was studied by comparing oxidative phosphorylation in the Saccharomyces cerevisiae cardiolipin synthase null mutant crd1Delta, and in isogenic wild type. Oxygen consumption experiments and membrane potential kinetics during the phosphorylation cycle in isolated mitochondria indicated that the absence of cardiolipin causes only a mod...