stay, Patient A received successively piperacillin/tazobactam with vancomycin, cefepime with ciprofloxacin, and imipenem with ciprofloxacin, but Patient B only received amoxicillin/clavulanate (Figure 1). It is well established that K. pneumoniae isolates are an important reservoir of b-lactamases. Thus, the dissemination of the KPC carbapenemase has been linked to the dispersion of a clonal ST...

INSERM U914 ‘Emerging Resistance to Antibiotics’, K.-Bicêtre, France; LabEx LERMIT, Faculté de Médecine Paris Sud, K.-Bicêtre, France; Centre National Associé-Centre de Référence des Résistances aux Antibiotiques, K.-Bicêtre, France; Medical and Molecular Microbiology, Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland; HFR-Hôpital Cantonal, Fribourg, Swit...

Klebsiella pneumoniae KpO3210 is a OXA-48 carbapenemase-producing isolate obtained from a blood culture in the context of an outbreak in Hospital Universitario La Paz (Madrid, Spain) in 2010. It belongs to the major clone detected during the outbreak and is resistant to all beta-lactams and to several other antibiotics.

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We present the draft genome sequence of a blood culture isolate of OXA-48-producing Klebsiella pneumoniae (sequence type 11 [ST11]) obtained in the course of a hospital outbreak in Spain. Sequence analysis showed 121 genes related to antibiotic and antiseptic resistance, including blaOXA-48, which was located in an IncL/M plasmid.

A chromosome-encoded beta-lactamase gene from a Shewanella oneidensis reference strain was cloned and expressed in Escherichia coli. It encoded a carbapenem-hydrolyzing Ambler class D beta-lactamase, OXA-54, that shared 92% amino acid identity with the plasmid-encoded carbapenem-hydrolyzing oxacillinase OXA-48 from Klebsiella pneumoniae. This work suggests that Shewanella spp. may produce the p...