The bacterial transposon Tn7 encodes five transposition genes tnsABCDE. We report a simple and rapid procedure for the purification of TnsC protein. We show that purified TnsC is active in and required for Tn7 transposition in a cell-free recombination system. This finding demonstrates that TnsC participates directly in Tn7 transposition and explains the requirement for tnsC function in Tn7 tra...

Long ridiculed for their racial hybridity, India’s Anglo-Indians—a mixed race people—were called ‘Dingos’ and instructed rudely to “Go to Australia” after India won her Independence from the British in 1947. Although their first port of escape from India was Great Britain, thousands of Anglo-Indians left the Indian subcontinent (India, Pakistan and Sri Lanka) in the second wave of migration, i....

Correspondence to: Beatriz Silva Câmara Mattos Faculdade de Odontologia Universidade de São Paulo Av. Lineu Prestes, 2227 05589-900 Cidade Universitária, São Paulo, SP Brazil Phone: (011) 55479547 Fax: (011) 51812229 E-mail: [email protected] Abstract Tooth transposition is an anomaly in the position of teeth where two teeth of the same maxillary quadrant change their position in the dental arch....

Snail, a zinc-finger transcription factor, controls the process of epithelial-mesenchymal transition, and ectopic expression of this protein may produce cells with stem cell properties. Because the effect of Snail expression in ovarian epithelial cells remains unclear, we generated Drosophila ovarian follicle stem cells (FSCs) with homozygous Scutoid (Sco) mutation. The Sco mutation is a recipr...

In Escherichia coli, the frequencies of IS50 and Tn5 transposition are greater in Dam- cells than in isogenic Dam+ cells. IS50 transposition is increased approximately 1,000-fold and Tn5 transposition frequencies are increased about 5- to 10-fold in the absence of Dam methylation. However, in cells that are deficient for both integration host factor (IHF) and Dam methylase, the transposition fr...

One possible model to study genome evolution is to represent genomes as permutations of genes and compute distances based on the minimum number of certain operations (rearrangements) needed to transform one permutation into another. Under this model, the shorter the distance, the closer the genomes are. Two operations that have been extensively studied are the reversal and the transposition. A ...

A genetic complementation system is described in which the complementing components are close together in a single linear DNA fragment; the complementation situation is temporary. This system is useful for providing transposition functions to transposition-defective transposons, since transposition functions act preferentially in cis. The basic procedure involves placing a transposition-defecti...