Objective. – To compare the effects of two cryoprotective agents (DMSO and 1,2-PROH) used at two concentrations (1,5 and 2 M) on the morphology of small ovarian cortex follicles in doe. Materials and methods. – Ovarian cortexes (n = 40) were frozen in TCM199 + 10% FCS medium added to 1.5 or 2 M of DMSO or 1,2-PROH. Two controls were realized (fresh and frozen without cryoprotectant). The equili...

BACKGROUND Cancer treatments, including chemotherapy, radiotherapy, and radical surgery, can induce premature menopause and infertility in hundreds of thousands of women of reproductive age every year. One of the ways to possibly preserve fertility before these treatments is to cryopreserve ovarian tissue for later transplantation. We aimed to restore fertility by cryopreservation and transplan...

BACKGROUND Some reports show that it is possible to isolate immature oocytes from human ovarian tissue retrieved by a cortex biopsy or ovariectomy of non-stimulated ovaries and mature them in vitro. The mature oocytes can be vitrified and stored for in vitro fertilization, which, along with ovarian tissue cryopreservation, is mostly practiced in young cancer patients to preserve their fertility...

Strategies to preserve fertility in dogs and cats as cryopreservation of epididymal spermatozoa, oocytes and ovarian tissue will be discussed in this review. Recent results indicate that significant progress have been achieved, but optimal freezing protocols have not been defined yet. The extreme sensitivity of sperm membranes and of oocyte and ovarian tissue structure deserves further investig...

There is currently much debate about cryopreservation of ovarian tissue or oocytes as a possible means of fertility preservation for women urgently needing potentially sterilizing medical treatment. Although both techniques are still experimental, some centres have started offering them also to healthy women who want to postpone childbearing until after they may have lost their natural reproduc...

Background Today, cryopreservation of ovarian tissue has been the effective procedure to restore fertility in can cer patients. It is well known that oxidative stress (OS) is a widespread phenomenon that occurs in ovary cryopreservation. Numerous studies show that follicular atresia in mammalian species due to the accumulation of toxic metabolites often results from oxidative stress. Therefore,...

Cryopreservation of oocytes and embryos is commonly used to preserve fertility. However, women undergoing cancer treatment may not have the time or may not be good candidates for these options. Ovarian cortical tissue cryopreservation and subsequent tissue transplant has been proven successful yet inefficient in preserving larger secondary follicles, and is not recommended as a fertility preser...

OBJECTIVE To determine the effect of storage duration on cryopreserved ovarian tissue using fresh and frozen-thawed samples. METHODS Seventeen fertile patients underwent an ovarian biopsy during elective laparoscopic tubal ligation. The tissue sample was divided into three parts: one part was processed fresh (FG), and two were slowly frozen, cryopreserved for 30 (G30) or 180 days (G180), thaw...

A surgical technique has been established for orthotopic transplantation of ovarian tissue in newly hatched chickens. In these trials survivability of the chicks after surgical manipulation was 100%. The size and orientation of the ovary at 2 wk of age suggested that the graft was attached and had undergone development. This technique should allow the development of a cryopreservation protocol ...

OBJECTIVE To compare the effects of dimethyl sulfoxide (DMSO), 1,2-propanediol (PROH), sucrose, trehalose, concentration of cryoprotectants, equilibration method, and postseeding freezing rate on doe rabbit ovarian tissue preservation after freezing, using fractional experimental design. DESIGN Experimental prospective study. SETTING Research institute in veterinary and agronomic colleges. ...