Received for publication, May 31, 2007, and in revised form, June 20, 2007 Published, JBC Papers in Press, June 21, 2007, DOI 10.1074/jbc.C700107200 Sandra E. Wiley, Mark L. Paddock, Edward C. Abresch, Larry Gross, Peter van der Geer , Rachel Nechushtai**, Anne N. Murphy, Patricia A. Jennings, and Jack E. Dixon From the Departments of Pharmacology, Physics, Chemistry and Biochemistry, and Cellu...

Introduction Hexokinase I binds to the mitochondrial surface of brain [l], muscle [2], fat [3], kidney [4] and liver cells [S]. Responsibility for this association lies with a specific binding protein [6], which has been identified as mitochondrial porin in the outer membrane [7,8] and is also known as voltage-dependent anion channel W A C ) [9]. The isolated outer membrane pore, when reconstit...

Heteronuclear NMR in combination with isotope labelling is used to study folding of polypeptides induced by metals the case metallothioneins, binding peptidic allosteric modulator ?-TIA human G-protein coupled ?1b adrenergic receptor, development therapeutic drugs that interfere biosynthesis outer membrane Gram-negative bacteria, and a system which protein assembly upon peptide addition. these ...

Most proteins in chloroplasts are encoded by the nuclear genome and synthesized in the cytosol. With the exception of most quter envelope membrane proteins, nuclear-encoded chloroplastic proteins are synthesized with N-terminal extensions that contain the chloroplast targeting information of these proteins. Most outer membrane proteins, however, are synthesized without extensions in the cytosol...

The Escherichia coli major outer membrane lipoprotein (Lpp) is released from the inner membrane into the periplasm as a complex with a carrier protein, LolA (p20), and is then specifically incorporated into the outer membrane. An outer membrane protein playing a critical role in Lpp incorporation was identified, and partial amino acid sequences of the protein, named LolB, were identical to thos...

Organelles are surrounded by membranes with a distinct lipid and protein composition. While it is well established that lipids affect protein functioning and vice versa, it has been only recently suggested that elevated membrane protein concentrations may affect the shape and organization of membranes. We therefore analyzed the effects of high chloroplast envelope protein concentrations on memb...

A native outer membrane vesicles (NOMV) vaccine was developed from three antigenically diverse strains of Neisseria meningitidis that express the L1,8, L2, and L3,7 lipooligosaccharide (LOS) immunotypes, and whose synX, and lpxL1 genes were deleted.. Immunogenicity studies in mice showed that the vaccine induced bactericidal antibody against serogroups B, C, W, Y and X N. meningitidis strains. ...

The biogenesis of mitochondria requires the import of a large number of proteins from the cytosol [1, 2]. Although numerous studies have defined the proteinaceous machineries that mediate mitochondrial protein sorting, little is known about the role of lipids in mitochondrial protein import. Cardiolipin, the signature phospholipid of the mitochondrial inner membrane [3-5], affects the stability...

The precursor of porin, a mitochondrial outer membrane protein, competes for the import of precursors destined for the three other mitochondrial compartments, including the Fe/S protein of the bc1-complex (intermembrane space), the ADP/ATP carrier (inner membrane), subunit 9 of the F0-ATPase (inner membrane), and subunit beta of the F1-ATPase (matrix). Competition occurs at the level of a commo...

Meningococcal disease is communicable by close contact or droplet aerosols. Striking features are high case fatality rates and peak incidences of invasive disease in infants, toddlers and adolescents. Vaccine development is hampered by bacterial immune evasion strategies including molecular mimicry.As for Haemophilus influenzae and Streptococcus pneumoniae, no vaccine has therefore been develop...