<p><strong>Background:</strong> Plant tissue culture has been shown to be an efficient technique for the propagation of diverse <em>Agave</em> species using different <em>in vitro</em> regeneration processes. However, it demonstrated that genetic changes can occur in plants regenerated under these schemes, also called somaclonal variation. <strong&gt...

An efficient in vitro regeneration protocol was developed via callus from the explants of leaf and internode Coccinia cordifolia (L.) Cogn., an important medicinal climber. Among different combinations auxins cytokines used, BAP with NAA were found highly effective for induction derived disc explants. Maximum formation rates recorded on MS 1.0 mg/l 0.1 both The best organogenesis obtained 2.0 0...