background: many studies reported that follicle size has an essential role in developmental potential of oocytes. also, the brilliant cresyl blue (bcb) test is one of the most important criteria in selection of more competent oocytes. objective: selection of developmentally competent bovine oocytes. materials and methods: a total of 1730 bovine cumulus oocyte complexes (cocs) were recovered fro...

OBJECTIVES We recently demonstrated spatial regionalization of maternal transcripts and proteins within unfertilized ovine oocyte. Here, we investigated the likelihood of oocyte polarity for the first time in bovine. MATERIALS AND METHODS In this experimental study, in vitro matured bovine oocytes were used for manual bisection [into oocyte halve that were near-to (HNS) and far-from (FS) spin...

Transcription factors in the germline play important roles in ovary formation and folliculogenesis, and control both oocyte development and somatic cell function. Factor in the germline (Figla) and newborn ovary homeobox gene (Nobox) represent a growing number of oocyte-specific transcription factors that regulate genes unique to oocytes. Studies on oocyte-specific transcription factors are imp...

In prepuberal mouse ovaries oocytes in the non-growing pool show constant but low levels of DNA-dependent RNA polymerase activity. The first event in the initiation of oocyte growth is a marked stimulation of RNA polymerase activity in the oocyte nucleolus. This is followed by increases in oocyte area, oocyte nucleolar area, oocyte nuclear area and nucleoplasmic RNA synthesis in that sequence. ...

Oocyte maturation is a complex process involving nuclear and cytoplasmic maturation. The nuclear maturation is a chromosomal segregation and the cytoplasmic maturation involves the reorganization of the cytoplasmic organelles, mRNA transcription and storage of proteins to be used during fertilization and early embryo development. The mechanism of oocyte maturation in vivo and in vitro still are...

OBJECTIVE To better understand women's beliefs, priorities, and attitudes toward oocyte cryopreservation, to appreciate the extent of their reproductive education, and to track the reproductive paths of women who chose to undergo oocyte cryopreservation treatment. DESIGN An anonymous 30-question survey. SETTING Not applicable. PATIENT(S) From 2005-2011, 478 women completed ≥1 oocyte cryop...

Increasing infertility is one of the most serious health problems today. Over past few years, we have had opportunity to follow progress technologies focused on production embryos in vitro (i.e., fertilization and intracytoplasmic sperm injection, genetic engineering, or somatic cell nuclear transfer. Oocyte maturation important processes vitro. Despite recent this field, developmental competen...

BACKGROUND Oocyte donation has been permitted by Swedish legislation since January 2003. While donors are anonymous to the receiving couple, offspring have the legal right to receive identifying information about the donor when they reach adult age. Our aim was to investigate factors of potential importance for women's willingness to donate oocytes. METHOD A questionnaire regarding attitudes ...

The assessment of oocyte quality in human in vitro fertilization (IVF) is getting increasing attention from embryologists. Oocyte selection and the identification of the best oocytes, in fact, would help to limit embryo overproduction and to improve the results of oocyte cryostorage programs. Follicular fluid (FF) is easily available during oocyte pick-up and theorically represents an optimal s...