Cancer may be detected at any age and could affect children, and reproductive age women as well. In recent years, cancer treatment has become less destructive and more specific. As a result, survival rates and quality of life following successful treatment have continuously improved. Cancer treatment typically involves surgery, chemo- or radiation therapy, or the combinations of these. These in...

this study investigated the effect of different harvesting techniques on the recovery rate and quality of bovine cumulus oocyte complexes (cocs) and their subsequent developmental competence including in vitro maturation, fertilization and blastocyst formation. the cocs were retrieved from slaughter ovaries using aspiration, slicing, or aspiration followed by slicing. the cocs were examined mic...

Since the introduction of human assisted reproduction, oocyte cryopreservation has been regarded as an attractive option to capitalize the reproductive potential of surplus oocytes and preserve female fertility. However, for two decades the endeavor to store oocytes has been limited by the not yet optimized methodologies, with the consequence of poor clinical outcome or of uncertain reproducibi...

Oocyte cryopreservation is a promising technology that could benefit women undergoing assisted reproduction. Most studies examining the effects of cryopreservation on fertilization and developmental competence have been done using metaphase II-stage oocytes, while fewer studies have focused on freezing oocytes at the germinal vesicle (GV) stage, followed by in vitro maturation. Herein, we exami...

The cryoloop is a technique where a thin nylon loop is used to suspend a film of cryoprotectant containing the oocytes and directly immersing them in liquid nitrogen. 508 bovine oocytes were collected, of these 351 were cryopreserved by slow freezing using standard straws or a new vitrification method using our self-constructed cryoloops and the remainder were controls. After thawing, the oocyt...

HE initial discovery by POLGE, SMITH and PARKES (1949) that glycerol aff ords protection to spermatozoa during freezing and thawing subsequently led to the development of semen banks which have greatly facilitated livestock improvement especially in the cattle breeding industry. However, until recently, the few attempts to preserve mammalian embryos by freezing have been relatively unsuccessful...

Over the past several decades, considerable effort has been expended toward the successful cryopreservation of various cells. While attempts at cryopreservation have been directed at different tissue types, one of the most vigorously pursued targets has been reproductive tissue. Historically, cryopreservation of sperm has existed for several decades. The earliest reports of pregnancies (Trounso...

During spermatogenesis, spermatozoa lose many of the organelles that most somatic cells possess (such as endoplasmic reticulum, lysosomes, and most of the cytoplasm) reducing the “baggage” they need to carry en route to the oocyte. The primary function of a spermatozoon, fertilizing an oocyte, is an integrated set of processes, which require multiple cell attributes. Therefore, although the spe...

To understand the application of artificial intelligence (AI), specifically Violet™ technology, in determining egg quality for women undergoing oocyte cryopreservation. Retrospective analysis at a fertility clinic. Patients 45yo and younger who underwent cryopreservation used Violet by Future Fertility from Mar 2020 to Apr 2021 were included. Cryopreservation statistics recorded. Oocyte outcome...