Polymerase chain reaction with confronting two-pair primers (PCR-CTPP) is a simple, timeand cost-effective SNP genotyping method. In this study, we propose a MA (memetic algorithm)-based method to design a feasible CTPP primer set. Overall, 288 SNPs in the SLC6A4 gene were tested in silico by the proposed method. The results indicate that the proposed method provides feasible CTPP primers that ...

cucumber mosaic virus (cmv) is one of the causal agents of viral diseases that infected mint (mentha spp.). cmv is a member of the cucumovirus genus in  bromoviridae family. the virus is naturally transmitted by aphids in a non-persistent manner.during  growing season 2007-2008, leaf samples from plants of mint fields with symptoms of viral disease were collected in mint fieldas in tehran provi...

In a West Nile virus (WNV) -free ecosystem, it is essential to verify the integrity of RNA before concluding that RNA extracted from mosquito specimens is negative for WNV gene sequences. The primary objective of our study was to develop a rapid molecular assay to rapidly screen mosquitoes for the presence of 18S RNA and WNV gene sequences. Mosquitoes, collected from multiple sites on the islan...

roots of helianthemum species were collected from various rangeland sites in fars, and other provinces in iran. the partial small subunits of ribosomal dna genes were amplified with the genomic dna extracted from their roots by nested polymerase chain reaction (pcr) using the universal fungal primer pair its1/its4 and specific primer pair ftc/rtc, which was designed based on internal transcribe...

It has long been presumed impossible to measure telomeres in vertebrate DNA by PCR amplification with oligonucleotide primers designed to hybridize to the TTAGGG and CCCTAA repeats, because only primer dimer-derived products are expected. Here we present a primer pair that eliminates this problem, allowing simple and rapid measurement of telomeres in a closed tube, fluorescence-based assay. Thi...

Sequence characterized amplifled region (SCAR) markers were derived from random amplified polymorphic DNAs (RAPDs) that segregate in a longleaf pine x slash pine Fi family. Nine RAPD fragments, five from longleafpine and four from slash pine, were cloned and end sequenced. A total of 13 SCAR primer pairs, with lengths between 17 and 24 nucleotides, were developed. Nine (for SCAR loci FGPO04, FG...

Ratoon stunting disease (RSD) of sugarcane, one of the most important diseases seriously affecting the productivity of sugarcane crops, was caused by the bacterial agent Leifsonia xyli subsp. xyli (Lxx). A TaqMan probe-based real-time quantitative polymerase chain reaction (qPCR) assay was established in this study for the quantification of Lxx detection in sugarcane stalk juice. A pair of PCR ...

The main objective of this study was to develop a reliable detection method for genetically modified cotton events MON 531 and MON 15985 by using multiplex PCR reaction technique. In this study we have used four primer pairs for the detection of individual gene segments present in transgene cassette of two GM cotton events which include CaMv35S promoter, Nos terminator, Cry1Ac and Npt II genes ...

A single step etch/primer that can be used for bonding orthodontic brackets with virtually any light polymerized resin-based composite bracket adhesive material that has been introduced. Clinical trials have shown it to be especially useful in re-bonding detached metallic or ceramic brackets, and for bonding brackets to fluorosed enamel. This technique report describes the PROMPT L-POP* system ...

anopheles fluviatilis complex is known to be a vector of malaria in iran. since mosquitoes of this species cover a wide geographical range in iran, they might have evolved into different separated populations. random amplified polymorphic dna polymerase chain reaction (rapd-pcr) assay was used to differentiate geographic populations of this species. dna was extracted from individual mosquitoes ...