Signal fluctuations in a fluorescence time trace on nanosecond time scales can be induced by specific quenching interactions that report on the dynamics of biomolecules. Fluorescence correlation spectroscopy is an analysis tool to investigate dynamic processes on time scales from pico- to milliseconds or longer. Under certain conditions, e.g. in a solvent of high viscosity, a fluorescence label...

Recently, considerable attention has been placed upon exploiting the negative-feedback effect in accelerating the quenching time of the avalanche current in passively quenched single-photon avalanche-diode (SPAD) circuits. Reducing the quenching time results in a reduction in the total charge generated in the SPAD, thereby reducing the number of trapped carries; this, in turn, can lead to impro...

We have applied an optimization method in conjunction with numerical simulations to minimize the mixing time of a microfluidic mixer developed for protein folding studies. The optimization method uses a semideterministic algorithm to find the global minimum of the mixing time by varying the mixer geometry and flow conditions. We describe the minimization problem and constraints and give a brief...

Gas quenching is a relatively new process with several important advantages, such as minimal environmental impact, clean products, and ability to control the cooling locally and temporally for best product properties. To meet the high cooling rates required for quenching, the cooling gas must flow at very high velocities, and such flows are highly turbulent and separated. Consequently, there is...

An absorptive chemically induced dynamic electron polarization (CIDEP) was generated by the quenching of singlet oxygen by nitroxide radicals (TEMPO derivatives). The spin polarization decay time of the nitroxide (measured by time-resolved EPR) correlates with the lifetime of singlet oxygen (measured by singlet oxygen phosphorescence spectroscopy). In addition, a deuterium isotope effect on the...