Studies have been made of the effects of nucleotides on binding of 1*61-glucagmm at its specitlc binding sites in plasma membranes of rat Ever. GTP and GDP, equally and at a minimal concentration of 0.05 m, stimulate the rate and degree of dissociation of bound labeled hormone, decrease uptake of glucagon by the membranes, and decrease the aflinity of the binding sites for glucagon. These effec...