Our current investigations on the chemical processes associated with muscular activity (1) require more sensitive methods for the determination of various metabolic intermediates than are currently available, and the present work is a contribution to the development of such procedures. These aim at the determination of hexose monophosphates on the one hand, fructose diphosphate and triose phosp...

A relatively important change in UV absorption is observed upon thermal perturbation of nucleotide solutions. Comparison of these thermal perturbation spectra of nucleic acid residues with solvent perturbation spectra of the same compounds suggests that this spectral change can most probably be attributed to temperature induced hydration change of the bases. This conclusion is confirmed by the ...

Background cAMP and cGMP have been known for a long time to be important second messengers in living cells, whereas other cyclic nucleotides like cCMP or cUMP have only been on the fringes of scientific interest. Recently, our group has discovered that bacterial adenylyl cyclase toxins produce a number of different cyclic nucleoside 5'-monophosphates (cNMPs) apart from cAMP, and the interest in...

Difluorinated cyclohexene diols (prepared from trifluoroethanol) can be elaborated to racemic analogues of phosphorylated sugars via regioselective protection and phosphorylation of the exposed C-1 hydroxyl group. Cis-diol protection was achieved using stannylene methodology, though the regioselectivity depended on the orientation of the methyl group at C-5. UpJohn dihydroxylation is effective ...

The gene dnph1 (or rcl) encodes a hydrolase that cleaves the 2'-deoxyribonucleoside 5'-monophosphate (dNMP) N-glycosidic bond to yield a free nucleobase and 2-deoxyribose 5-phosphate. Recently, the crystal structure of rat DNPH1, a potential target for anti-cancer therapies, suggested that various analogs of AMP may inhibit this enzyme. From this result, we asked whether N (6)-substituted AMPs,...

L-glutamate, typically as its Na salt (MSG), elicits a taste termed umami. A characteristic feature of umami taste is the synergistic potentiation of glutamate taste by purine nucleotide (inosine, guanosine) monophosphates. This is manifested as an enhanced electrophysiological response from taste receptor cells, as an increase in nerve firing rate, or as increased preference in behavioral assa...

Background The cyclic nucleotide monophosphates cGMP and cAMP play an essential role in many signaling pathways. The identification and profiling of cGMPand cAMP-binding proteins is an important step in order to elucidate the molecular basis of these pathways. In order to develop a tool to specifically target cGMPand cAMP-binding proteins, we synthesized cGMPand cAMP Capture CompoundsTM (CCs). ...

The nucleotidyl cyclase toxin ExoY is one of the virulence factors injected by the Pseudomonas aeruginosa type III secretion system into host cells. Inside cells, it is activated by an unknown eukaryotic cofactor to synthesize various cyclic nucleotide monophosphates. ExoY-like adenylate cyclases are also found in Multifunctional-Autoprocessing Repeats-in-ToXin (MARTX) toxins produced by variou...