By transplanting nuclei between labeled and unlabeled cells, we determined the localization of the major proteins of amebas and described certain features of their intracellular distributon. We identified approximately 130 cellular proteins by fluorography of one-dimensional polyacrylamide electrophoretic gels and found that slightly less than half of them (designated NP, for nuclear proteins) ...

BACKGROUND Somatic cell nuclear transfer (SCNT) requires cytoplast-mediated reprogramming of the donor nucleus. Cytoplast factors such as maturation promoting factor are implicated based on their involvement in nuclear envelope breakdown (NEBD) and premature chromosome condensation (PCC). Given prior difficulties in SCNT in primates using conventional protocols, we hypothesized that the ability...

Isotopic and nuclear techniques play an important role in food and agriculture, health and industry. In the agricultural sciences and food technology sectors, recent research has elucidated the new potential application of radiation for microbial decontamination, food preservation, improve the nutritional quality of  foods  and effectively utilise its potential as human food or animal feed. The...

solitary pulmonary nodule (spn) is a frequent finding on the chest x-ray and computed tomography. nuclear medicine techniques play an important role in the diagnosis and management of spn. in the current review, we briefly will explain the different nuclear medicine modalities in this regard including positron emission tomography (pet) using 18-f-fdg, and 11-c-methionine, and single photon emis...

We introduce a broadly applicable technique to create nuclear spin singlet states in organic molecules and other many-atom systems. We employ a novel pulse sequence to produce a spin-lock induced crossing (SLIC) of the spin singlet and triplet energy levels, which enables triplet-singlet polarization transfer and singlet-state preparation. We demonstrate the utility of the SLIC method by produc...