The genome of influenza A virus is organized into eight ribonucleoprotein complexes (RNPs), each containing one RNA polymerase complex. This RNA polymerase has also been found non-associated to RNPs and is possibly involved in distinct functions in the infection cycle. We have expressed the virus RNA polymerase complex by co-tranfection of the PB1, PB2 and PA genes in mammalian cells and the he...

Recent studies have uncovered an unanticipated diversity of noncoding RNAs (ncRNAs), although these studies provide limited insight into their biological significance. Numerous general methods for identification and characterization of protein interactions have been developed, but similar approaches for characterizing cellular ncRNA interactions are lacking. Here we describe RNA Affinity in Tan...

Regional Nature Parks (RNPs) such as the Parc Ela and Beverin Park are popular destinations for outdoor activities recreation in Alpine areas of Switzerland. As many other mountain peripheral regions, their soundscapes being increasingly influenced by humans. Little is known about which sound types perceived positively or negatively visitors how sounds affect visitors’ recreational quality. T...

Cajal bodies (CBs) have been implicated in the nuclear phase of the biogenesis of spliceosomal U small nuclear ribonucleoproteins (U snRNPs). Here, we have investigated the distribution of the CB marker protein coilin, U snRNPs, and proteins present in C/D box small nucleolar (sno)RNPs in cells depleted of hTGS1, SMN, or PHAX. Knockdown of any of these three proteins by RNAi interferes with U s...

Determining the in vivo targets of RNA-binding proteins and characterizing the posttranscriptional networks in which they participate constitute major challenges in the post-genomic era. An important step in this direction is the development of methods that permit efficient recovery of ribonucleoprotein (RNP) complexes. We present an improved methodology for efficient isolation of mammalian cel...

tors by the specific names used in their original descriptions. These receptors are generally large (90–130 kDa) Philadelphia, Pennsylvania 19104-6148 acidic proteins sharing 15%–25% sequence identity within a given organism. They all have an N-terminal RanGTP-binding domain, a C-terminal cargo-binding Eukaryotic cells are equipped with a machinery charged domain, and the capacity to bind compo...

The spliceosome is a mega-Dalton ribonucleoprotein (RNP) assembly that processes primary RNA transcripts, producing functional mRNA. The electron microscopy structures of the native spliceosome and of several spliceosomal subcomplexes are available; however, the spatial arrangement of the latter within the native spliceosome is not known. We designed a computational procedure to efficiently fit...

RNPs: Ribonucleoproteins; snRNPs: Small Nuclear Ribonucleoproteins; snRNA: Small Nuclear RNA; BP: Branch Point; PPT: Poly Pyrimidine Tract; ISE: Intronic Splicing Enhancer; ESE: Exonic Splicing Enhancer; ISS: Intronic Splicing Silencer; ESS: Exonic Splicing Silencer; SR Proteins: Serine/ArginineRich Proteins; RRM: RNA-Recognition Motif; RS Domains: Arginine and Serine Rich Domains; hnRNPs: Hete...

The signal requirement for the nuclear import of U1 RNA in somatic cells from different species was investigated by microinjection of both digoxygenin-labeled wild type and mutant U1 RNA molecules and in vitro reconstituted U1 snRNPs. U1 RNA was shown to be targeted to the nucleus by a temperature-dependent process that requires the prior assembly of RNPs from the common proteins and the microi...

Abstract Background To meet increasing demand for forest-based products and protect natural forests from further deforestation requires increased productivity planted forests. Genetic improvement of conifers by traditional breeding is time consuming due to the long juvenile phase genome complexity. modification (GM) offers opportunity make transformational changes in shorter frames but challeng...