Neurotrophin-3 (NT-3) promotes enteric neuronal development in vitro; nevertheless, an enteric nervous system (ENS) is present in mice lacking NT-3 or TrkC. We thus analyzed the physiological significance of NT-3 in ENS development. Subsets of neurons developing in vitro in response to NT-3 became NT-3 dependent; NT-3 withdrawal led to apoptosis, selectively in TrkC-expressing neurons. Antibodi...

DNA methylation is an important factor for the regulation of gene expression in early embryos. It is well known that the satellite I sequence is more heavily methylated in bovine somatic cell nuclear transfer (NT-SC) embryos than in embryos derived from in vitro fertilization (IVF). However, the methylation status of bovine embryos obtained by other procedures is not well known. To clarify DNA ...

This paper is intended to catalyze discussions on two intertwined systems topics. First, it presents early results from a latency study of Windows NT that identifies ome specific auses of long thread scheduling latencies, many of which delay the dispatching of runnable threads for tens of milliseconds. Reasons for these delays, including technical, methodological, and economic are presented and...

Imperialism in New Testament (related) films relates to an important aspect of the reception of the Bible in film. After a brief investigation of the concept imperialism, this contribution explores particular instances where imperialism and NT themes intersect in film: the relationship between imperialism, Jesus-followers and Romans; satirical approaches to imperialism in NT films; and, the int...

The word "mystery" as it occurs in ancient Greek and Semitic sources. as well as in the NT. refers to a secret which is only revealed to 'certain individuals. In the NT it is God who reveals the mystery and faithful believers who perceive it. Carnal believers and unbelievers are not able to understand the mystery. The mystery centers around the Lordship of Christ who is the Life of the church. ...

Two different sizes of siRNAs, of which one type was 21-nucleotide (nt) siRNA containing 2-nt dangling ends and the other type was 27-nt siRNA with blunt ends, were conjugated with a nuclear export signal peptide of HIV-1 Rev at the 5'-sense end. Processing by Dicer enzyme, cell membrane permeability, and RNAi efficiency of the peptide-conjugated siRNAs were examined. Dicer cleaved the peptide-...

BACKGROUND The frequent expression of neurotensin receptors (NT-R) in primaries of pancreatic ductal carcinomas has triggered the development of radioactive neurotensin analogs for possible in vivo targeting of these tumors. However, the complete lack of information regarding NT-R in liver metastases of pancreatic cancer and pancreatic intraepithelial neoplasia (PanIN) makes an in vitro study o...

OBJECTIVE To investigate the novel hypothesis that neurotrophin-3 (NT-3), an established neurotrophic factor that participates in embryonic heart development, promotes blood vessel growth. METHODS AND RESULTS We evaluated the proangiogenic capacity of recombinant NT-3 in vitro and of NT-3 gene transfer in vivo (rat mesenteric angiogenesis assay and mouse normoperfused adductor muscle). Then, ...