Chitosan has been indicated as a safe and promising polycation vector for gene delivery. However its low transfection efficiency has been a challenging obstacle for its application. To address this limitation, we synthesized chitosan derivatives which had increasing amounts of diethylethylamine groups (DEAE) attached to the chitosan main chain. The plasmid DNA VR1412 (pDNA), encoding the ß-gala...

Urease belongs to a family of highly conserved urea-hydrolyzing enzymes. A common feature of these enzymes is the presence of two Lewis acid nickel ions and reactive cysteine residue in the active sites. In the current study we examined a series of biscoumarins 1-10 for their mechanisms of inhibition with the nickel containing active sites of Jack bean and Bacillus pasteurii ureases. All these ...

The purpose of the present study was to develop immunotoxins directed against human ovarian carcinoma cells. Four monoclonal antibodies (260F9, 454C11, 280D11, and 245E7) were chosen because they were found to bind to various ovarian carcinoma cell lines. These antibodies were covalently linked to either Pseudomonas exotoxin (PE) or ricin A chain (RTA), and the conjugates were tested against fi...

PURPOSE Tissue adhesion is a well-known postsurgical phenomenon, causing pain, functional obstruction, and difficult reoperative surgery. To overcome these problems, various synthetic and natural polymer membranes have been developed as postoperative tissue adhesion barriers. However, limitation in their use has hindered its actual application. We prepared a hyaluronate membrane (HM) to evaluat...

Since penicillin became available for the treatment of bacterial infections, the nontoxicity of the drug has been the subject of universal emphasis. Nevertheless, a number of toxic sequels, chiefly of minor importance, have been reported. These were more frequent in the early days of penicillin therapy, have gradually become less common, and were probably correctly ascribed, in the majority of ...

INTRODUCTION Emulsion polymerization yielding polymer latexes, is carried out on a vast scale. The use of water as a dispersion medium offers a unique combination of features, such as effective heat transfer, effective stabilization of polymer particles by surfactants, and environmental friendliness. By contrast to polymerization in solution or in the bulk, in emulsion polymerization a large po...

CeO2 nanoparticles have been proven to be competent photocatalysts for environmental applications because of their strong redox ability, nontoxicity, long-term stability, and low cost. We have synthesized CeO2 nanoparticles via solution combustion method using ceric ammonium nitrate as an oxidizer and ethylenediaminetetraacetic acid (EDTA) as fuel at 450 °C. These nanoparticles exhibit good pho...

TiO2 is an exemplary semiconductor anode material for photoelectrochemical (PEC) water-splitting electrodes due to its functionality, long-term stability in corrosive environments, nontoxicity, and low cost. In this study, TiO2 photoanodes with enhanced photocurrent density were synthesized by atomic layer deposition (ALD) of TiO2 onto a porous, transparent, and conductive fluorine-doped tin ox...

Most widely used carbon-based materials feature a bulk framework tolerant of extreme pH and temperature conditions while maintaining physical robustness, electrical conductivity, biological relevance due to its inherent nontoxicity. At the nanoscale, study porous carbons has been interest because enhanced accessible surface area relative nonporous analogues. The evolution synthetic techniques d...

Chitosan is a linear polysaccharide with prominent physicochemical and biological properties such as biocompatibility, biodegradability, nontoxicity, nonimmunogenicity, bioadhesion, antibacterial, antifungal, hemostatic activity. Due to these properties, it has found many applications in cosmetic, textile, food industries; agriculture; biotechnology; pharmaceutical industry medicine, especially...