Background Degenerative retinal diseases, including age related macular degeneration, glaucoma, and hereditary retinal dystrophies are major causes of blindness. The principal defect in these diseases is cell loss which is amenable to both cell based neuroprotective and neuroregenerative therapies. To briefly review the lines of research and potential candidates for cell based therapies among ...

Embryonic stem cells (ESCs) are key tools for genetic engineering and development of stem cell-based therapies. The investigation of ESCs pluripotency and early lineage commitment is an essential precondition for its translational use. In this study, we explored the ability of mouse ESC to differentiate into inner ear hair cell lineages. Mouse embryonic stem cells (E14TG2A) were cultured on pri...

background: bone marrow has recently been recognized as a novel source of stem cells for the treatment of wide range of diseases. a number of studies on murine bone marrow have shown a homogenous population of rare stage-specific embryonic antigen 1 (ssea-1) positive cells that express markers of pluripotent stem cells. this study focuses on ssea-1 positive cells isolated from murine bone marro...

Stem cells are undifferentiated cells that are capable of dividing for long periods of time and can give rise to specialized cells under particular conditions. Embryonic stem cells [5] are a particular type of stem cell derived from embryos. According to US National Institutes of Health [6] (NIH), in humans [7], the term ?embryo? applies to a fertilized egg [8] from the beginning of division up...

abstract introduction: cancer stem cells are a small subpopulation of cells within a tumor which are responsible for maintaining the tumor mass. a number of factors such as oct-4 that govern the fate of adult stem cells also play a role in malignant cell transformation. oct-4 is a key regulator of self-renewal in embryonic stem cells; its expression is potentially correlated with tumorigenesis ...

To find a suitable feeder layer is important for successful culture conditions of bovine embryonic stem cell-like cells. In this study, expression of pluripotency-related genes OCT4, SOX2 and NANOG in bovine embryonic stem cell-like cells on mouse embryonic fibroblast feeder layers at 1-5 passages were monitored in order to identify the possible reason that bovine embryonic stem cell-like cells...

BACKGROUND Several studies have demonstrated the derivation of multi- or pluripotent stem cells from testicular cells of both newborn and adult mice by a spontaneous conversion process, when these cells are cultured in vitro for an extended time. To obtain a better and robust derivation, we have attempted to identify small molecules (SMs) that induce reprogramming of testicular cells in culture...

aims: to evaluate the effect of mouse fallopian tube cell co-culture on the proliferation of human endometrial stromal cells. materials and methods: the endometrial cells were collected from the uterus under hysteroscopy. then the cells were isolated withcollagenase type 3 and passed within the filters with 150 and 40 micrometer sizes respectivelyand then the isolated cells were cultured in dme...