During myocardial reperfusion, polymorphonuclear neutrophil (PMN) adhesion involving the intercellular adhesion molecule-1 (ICAM-1) may lead to aggravation and prolongation of reperfusion injury. We studied the role of early tumor necrosis factor-a (TNF-a) cleavage and nuclear factor-kB (NF-kB) activation on ICAM-1 expression and venular adhesion of PMN in isolated hearts after ischemia (15 min...

BACKGROUND: Cisplatin is one of the most potent chemotherapy drugs to treat various types cancer, however use cisplatin has some adverse effect, such as increase oxidative stress and inflammation by malondialdehyde (MDA) nuclear factor kappa B (NF-kB) activation. Since dosing critical, we observed effect single multiple doses injection on rats’ level.METHODS: Total 27 male Sprague-Dawley rats w...

Living cells must be capable of rapidly responding to intrinsic and environmental cues to ensure normal development and homeostasis. The NF-jB transcription factor mediates a series of such rapid-response programs, serving as a master regulator of gene transcription in cells subjected to inflammation, irradiation, or other stress states (Karin, 2009). While cells must be able to rapidly respond...

We have read the article with interest by Zhuang and colleagues (1) on the combination of IL1R antagonist and gemcitabine in gemcitabine-resistant pancreatic ductal adenocarcinoma (PDAC). The article showed that gemcitabine-induced NF-kB and ERK activation in gemcitabine-resistant PDAC cells can be reversed by anakinra, an FDA-approved IL1R antagonist. However, there are still some key points t...

APPL1 is a multifunctional adaptor protein that binds membrane receptors, signaling proteins and nuclear factors, thereby acting in endosomal trafficking and in different signaling pathways. Here, we uncover a novel role of APPL1 as a positive regulator of transcriptional activity of NF-kB under basal but not TNFa-stimulated conditions. APPL1 was found to directly interact with TRAF2, an adapto...

Compounds isolated from members of the Zingiberaceae family are traditionally used as a medicine against inflammatory diseases, but little is known about the mechanism. Here, we report the isolation and structural identification of coronarin D [E-labda-8(17),12-diene-15-ol], a labdane-type diterpene, from Hedychium coronarium and delineate its mechanism of action. Because the transcription fact...

We and others have recently shown that soyasaponins abundant in soybeans can decrease inflammation by suppressing the nuclear factor kappa B (NF-kB)-mediated inflammation. However, the exact molecular mechanisms by which soyasaponins inhibit the NF-kB pathway have not been established. In this study in macrophages, soyasaponins (A1, A2 and I) inhibited the lipopolysaccharide (LPS)-induced relea...

In the recently published Molecular Pathways article (1), Molineaux summarized the recent progress in the development of proteasome inhibitors for the treatment of cancer. The author stated that inhibition of NF-kB is one of the major mechanisms mediating the antitumor effect of proteasome inhibition. This notion has been supported by the finding that proteasome is responsible for the degradati...

PURPOSE To explore the function of DREAM gene mediated by NF-kB signal pathway in the pathogenesis of osteosarcoma. METHODS This study included 13 Sprague Dawley (SD) rats with osteosarcoma (treatment group) and 13 healthy rats (control group). NF-kB, DREAM and P105 mRNAs expression levels were determined using quantitative PCR (qPCR). The expression levels of NF-kB, DREAM and P105 proteins w...

The expression of the gene encoding tumor necrosis factor /3 (TNF-P) (lymphotoxin) is induced in T cells by various extracellular stimuli. We noticed that most such stimuli also activate the NF-KB transcription factor. Here we demonstrate binding of purified human NF-KB to a sequence within positions 98 to -88 (S-GGGGCTTCCCC-3’) of the TNF-P promoter, which is conserved between the human and mo...