recently pcr is being used more frequently as a diagnostic method to detect m. pneumoniae. we used primer pairs reported by van kuppeveld,13 leng,5 lunerberg,7 an bernet' targeting 16s rrna, pi protein, tuf genes. and a short dna equence (mp5) to evaluate the sensitivity among different pcrs. reoptimization experiments showed that tuf pcr had the highest sensitivity amongst these four pcrs...

Dear Editor, The increasing incidence of aspergillosis emphasizes to improve the diagnostic tools. 1 Aspergillus fumigatus is associated with a high rate of mortality among patients receiving Bone Marrow Transplantation (BMT). Diagnosis in the early stages of invasive aspergillosis (IA) is very difficult, as clinical and radiological sings are nonspecific. 2 In this study, we used nested PCR te...

Accurate and rapid diagnosis of malaria infections is crucial for implementing species-appropriate treatment and saving lives. Molecular diagnostic tools are the most accurate and sensitive method of detecting Plasmodium, differentiating between Plasmodium species, and detecting subclinical infections. Despite available whole-genome sequence data for Plasmodium falciparum and P. vivax, the majo...

PCR assays for the diagnosis of systemic candidiasis can be performed either on serum or on whole blood, but results obtained with the two kinds of samples have never been formally compared. Thus we designed a nested PCR assay in which five specific inner pairs of primers were used to amplify specific targets on the rRNA genes of Candida albicans, C. tropicalis, C. parapsilosis, C. krusei, and ...

A nested-PCR temporal temperature gradient gel electrophoresis (TTGE) approach was developed for the detection of bacteria belonging to phylogenetic cluster I of the genus Clostridium (the largest clostridial group, which represents 25% of the currently cultured clostridial species) in cheese suspected of late blowing. Primers were designed based on the 16S rRNA gene sequence, and the specifici...

Molecular mechanisms related to occult hepatitis B virus (HBV) infection, particularly those based on genotype C infection, have rarely been determined thus far in the ongoing efforts to determine infection mechanisms. Therefore, we aim to elucidate the mutation patterns in the surface open reading frame (S ORF) underlying occult infections of HBV genotype C in the present study. Nested PCRs we...

Endoscopy-related infections are important contributors to nosocomial infections. Endoscope reprocessing according to standard guidelines ensures high-level disinfection and prevents endoscopy-related infections. Microbiological surveillance may help in monitoring the effectiveness of gastrointestinal endoscope disinfection. The process involves microbial cultures and non-culture methods such a...

Recently PCR is being used more frequently as a diagnostic method to detect M. pneumoniae. We used primer pairs reported by Van Kuppeveld,13 Leng,5 Lunerberg,7 an Bernet' targeting 16s rRNA, PI protein, tuf genes. and a short DNA equence (MP5) to evaluate the sensitivity among different PCRs. Reoptimization experiments showed that tuf PCR had the highest sensitivity amongst these four PCRs, det...