Neospora caninum, an obligate intracellular protozoan parasite, can infect a large variety of vertebrate hosts including the most economically important cattle. Infection with N. caninum is a main cause of abortion in both dairy and beef cattle, which causes great economic losses worldwide. However, the mechanism of host cell infection by N. caninum has not been fully elucidated, especially in ...

The enzyme-linked immunosorbent assay (ELISA) was used to examine cross-reactivity of Neospora caninum with Toxoplasma gondii and Hammondia heydorni. Anti-T. gondii mouse and cat sera cross-reacted with N. caninum soluble antigen (NLA), but not with the recombinant surface antigen (NcSRS2). Anti-H. heydorni dog sera showed no cross-reactivity with either the NLA antigen or the NcSRS2. Lack of c...

The role of rodents in the epidemiology of neosporosis was investigated by assaying brain tissue of feral mice (Mus musculus) and rats (Rattus norvegicus) for Neospora caninum. Both mouse and rat brain tissue were extracted for total DNA, and subjected to two different N. caninum-specific nested polymerase chain reaction (PCR) assays. A portion of brain tissue from the mice and rats were also a...

neospora caninum is a coccidian protozoan that causes abortion in dairy and beef cattle and neurological disorders in dogs and horses. to identify n. caninum oocysts in the dog feces the molecular approaches are known as sensitive methods that specifically detect the oocysts. in present study, a polymerase chain reaction (pcr) targeting n. caninum specific nc5 genomic fragment was performed to ...

We report the development of a real-time PCR assay for the quantitative detection of Neospora caninum in infected host tissues. The assay uses the double-stranded DNA-binding dye SYBR Green I to continuously monitor product formation. Oligonucleotide primers were designed to amplify a 76-bp DNA fragment corresponding to the Nc5 sequence of N. caninum. A similar method was developed to quantify ...

Aim: The aim of present study was to determine the seroprevalence of Neospora caninum infection in aborted sheep population from Hamedan province of Iran. Materials and Methods: 358 serum samples of aborted ewes were evaluated for IgG-antibodies to N. caninum using enzymelinked immunosorbent assay (ELISA). Results: 8 samples (2.2%) were found seropositive for this infection (2.05< Confidence in...

The purpose of this study was to investigate whether sporulated Neospora caninum oocysts, which had been stored for 46 mo in a 2% sulfuric acid solution at 4 degrees C, remain morphologically viable and infective to gerbils (Meriones unguiculatus). Six gerbils were orally inoculated with doses of 400 or 1,200 oocysts. Two mo after inoculation, the animals did not show any clinical signs, had no...

Neospora caninum is a parasite regarded a major cause of foetal loss in cattle. A key requirement to an understanding of the epidemiology and pathogenicity of N. caninum is knowledge of the biological characteristics of the species and the genetic diversity within it. Due to the broad intermediate host range of the species, worldwide geographical distribution and its capacity for sexual reprodu...

Neosporosis is caused by the protozoan parasite, Neospora caninum which is major cause of fatal disease and abortion in cattle in Iran and in many countries. The economic impacts of neosporosis are lost milk, meat and abortions in cattle. The purpose of the present study was to evaluate the presence of N. caninum DNA in fresh and frozen semen samples of Iranian bulls that were used for artifici...

Background: Neosporosis is a parasitic disease caused by Neospora caninum. This parasite an obligate intracellular coccidia similar to Toxoplasma gondii with global distribution. With regard the experimental studies, vertical transmission of in monkey (non-human primates) has increased concern about zoonotic potential this disease. The principal aim current research was perform mini-review on i...