The New Delhi metallo-β-lactamase (NDM-1) is involved in the emerging antibiotic resistance problem. Development of metallo-β-lactamases (MBLs) inhibitors has proven challenging, due to their conformational flexibility. Here we report site-selective labeling of NDM-1 with 1,1,1-trifluoro-3-bromo acetone (BFA), and its use to study binding events and conformational changes upon ligand-metal bind...

Naturalistic decision making (NDM) has become established as a methodological and theoretical perspective. It describes how practitioners actually make decisions in complex domains. However, NDM theories tend to focus on the human agents in the system. We extend the NDM perspective to include the technological agents in complex systems and introduce the dynamic model of situated cognition. We d...

The emergence of one of the most recently described carbapenemases, namely, the New Delhi metallo-lactamase (NDM-1), constitutes a critical and growingly important medical issue. This resistance trait compromises the efficacy of almost all lactams (except aztreonam), including the last resort carbapenems. Therapeutical options may remain limited mostly to colistin, tigecycline, and fosfomycin. ...

Commercial manufacturing of dairy products involves the addition ingredients (such as nonfat dry milk and protein concentrates) well non-dairy additives gums, stabilizers, emulsifiers, texture modifiers) to get best product appearance, maintain quality, extend shelf-life. Though these are not harmful, consumers do prefer them in food formulations. Therefore, industry is working on improving inh...

Neonatal diabetes mellitus (NDM) is a rare kind of diabetes characterized by hyperglycemia and low levels of insulin. Clinically, it is categorized into two main types: transient NDM (TNDM) and permanent NDM (PNDM). These types are diagnosed based on duration of insulin dependence early in the disease. In TNDM, diabetes begins in the first few weeks of life with remission in a few months. Howev...

The NDM-1 gene, first identified in Sweden in 2008 in Klebsiella pneumoniae from a patient hospitalized in New Delhi, encodes a metallo-β-lactamase that inactivates all β-lactams except aztreonam. This bla(NDM-1) gene has been identified in hospital-acquired bacterial species, such as K. pneumoniae, but also in the typical community-acquired species, Escherichia coli. This gene has been identif...

Correspondence Emergence of an Autochthonous and Community-Acquired NDM-1–Producing Klebsiella pneumoniae in Europe To the Editor—The recently identi-tified carbapenemase New Dehli metallo-b-lactamase (NDM-1) in-activates all b-lactams except az-treonam [1]. The corresponding gene that is usually plasmid-borne has spread mostly in Escherichia coli and Klebsiella pneumoniae [1, 2]. NDM-1 produce...

BACKGROUND We have recovered one bla(NDM-1)-harboring bacterial strain, designated as XM1570, from a sputum sample obtained from a fatal case of pneumonia in China. METHODS Biochemical profiling, 16S rRNA sequencing and antimicrobial susceptibility testing were performed. Conjugation experiments were conducted to determine transmissibility of resistance. Pulsed-field gel electrophoresis and w...

OBJECTIVES The objective of this study was to characterize the molecular mechanism of coproduction of KPC-2 and NDM-1 in Citrobacter freundii. METHODS C. freundii strain 112298 was isolated from a human case of septic shock in a Chinese teaching hospital. The major carbapenemase and ESBL genes were detected by PCR. The MIC values were determined by using VITEK 2 and antimicrobial susceptibili...

The naturalistic decision making (NDM) approach seeks to understand human cognitive performance by studying how individuals and teams actually make decisions in real-world settings. Three major criteria have appeared in the literature to describe research that counts as NDM study: The research focuses on expertise, it takes place in field settings, and it reflects the conditions (such as uncert...