Understanding membrane protein folding and stability is required for a molecular explanation of function and for the development of interventions in membrane protein folding diseases. Stable aqueous detergent solutions of the Escherichia coli glycerol facilitator in its native oligomeric state have been difficult to prepare as the protein readily unfolds and forms nonspecific aggregates. Here, ...

The increasing adoption of Graphic Process Unit (GPU) to computation-intensive workloads has stimulated a new computing paradigm called GPU cloud (e.g., Amazon’s GPU Cloud), which necessitates the sharing of GPU resources to multiple tenants in a cloud. However, state-ofthe-art GPU virtualization techniques such as gVirt still suffer from non-trivial performance overhead for graphics memory-int...

Electrophoregrams with bands of green chlorophyll-containing protein complexes separated by native polyacrylamide gel electrophoresis (PAGE) are usually photographed in white light or scanned by a narrow beam of monochromatic light using densitometers. Present CCD cameras allow taking densitograms of a whole gel surface with many samples. However, commercial CCD imaging systems use mostly white...

The two-dimensional blue native/sodium dodecyl sulfate polyacrylamide gel electrophoresis (2D BN/SDS-PAGE) is a method of choice for the investigation of protein complexes. This highly resolvent separation method is unique in that it facilitates the identification of many protein complexes simultaneously. Because of its simplicity and suitability, the 2D BN/SDS-PAGE can be now applied to a wide...

A multicomponent protein complex containing manganese (II)-dependent peroxidase, laccase and beta-glucosidase was isolated from culture extracts of the white rot basidiomycete Lentinula edodes. This protein complex showed a single protein band on native polyacrylamide gel electrophoresis (PAGE). On sodium dodecyl sulfate (SDS)-PAGE, however, it displayed three major bands and several additional...

The chemical structure of (−)-isoscopariusin A (at the top) isolated from Isodon scoparius is shown, with White-Water Terraces (Baishuitai), native region species in Yunnan Province China, background. flowers and leaves are shown inset. On right side, sclareolide as starting material. Details synthesis reported Research Article by Ang Li, Pema-Tenzin Puno et al. on page 12859. Graphene Bioanaly...