The gerbera market would benefit from an efficient and simple protocol for high rate regeneration for propagation and genetic engineering. With these objectives, this investigation was done on shoot regeneration via direct organogenesis from leafy petiole explants of Gerbera jamesonii ‘Royal Soft Pink’. Murashige and Skoog (1962) (MS) medium was supplemented with 0.1 mg L-1 indole-3-acetic acid...

In vitro mass production of C. suaveolens (Lindl.) Hook, an endangered orchid with its snowy white flowers having horticultural potential was accomplished through immature seed culture, and subsequent plant regeneration. The developmental stage of the immature seeds and nutrient media significantly influenced the germination frequency. Seeds at 13 months after pollination cultured on 3% sucrose...

alpha-Naphthaleneacetic acid-C(14), labeled in the carboxyl group, was applied in blocks of agar to the distal and to the proximal (either apical or basal) ends of explants of Coleus. The radioactivity in receiver blocks at the opposite ends was measured. Acropetal transport was slight, only 4% of the basipetal transport.Translocation of NAA-C(14) was polar in basipetal direction. Only 1.4% of ...

Strawberry (Fragaria × ananassa Duch.) can be easily propagated with daughter plants or through crown division, which are developed from the axillary bud at axils of leaves. This study was conducted to investigate effects different cytokinins, auxins, and their combinations on growth in strawberry. Four cytokinins (6-benzyladenine, kinetin, zeatin, thidiazuron (TDZ)) three auxins (indole-3-acet...

By repeated selection of pigment portions of tissue the red callus induced from root seed­ lings of Penstemon serrulatus Menz. was chosen for suspension culture, which was maintained in Schenk and Hildebrandt medium supplemented with naphthaleneacetic acid (0.2 mg/1), 6-benzylaminopurine (2 mg/1) and sucrose (50 g/1). From the cultured cells eight phenolic compounds were isolated. They were ide...

Leaves and stems of CephalolaxlIs harringlonia were induced to callus on Murashige and Skoog medium supplemented with additional vitamins, hypoxanthine, naphthaleneacetic acid and kinetin. Analysis by GC-MS showed cephalotaxine and its antitumor esters (harringtonine, isoharringtonine and homoharringtoninel in both callus and medium, whereas deoxyharringtonine was present (in relatively large a...

Ecophysiological stimulators directly affect root morphology, especially in the embryonic stage. To enhance crop germination, an understanding of traits under abiotic inducers is needed. In this study, combined impacts white and red-blue light spectrums, cold stratification, seed fortification involving various concentrations bioactive chemicals namely simple nutrient addition program solution,...

This paper presents a convenient method for the determination of 1-naphthaleneacetic acid (NAA) plant growth regulator in spiked canned pineapple samples by micellar-stabilized room temperature phosphorescence with sodium dodecyl sulfate as a micellar medium, thallium nitrate as an external heavy atom, and sodium sulfite as an oxygen scavenger. A multivariate optimization approach using the typ...