The effects of temperature upon the respiratory pathways of Arum maculatum mitochondria have been studied. The alternate oxidase sustained a greater proportion of the total respiration at low temperatures than at higher temperatures. Arrhenius plots of respiratory activities show two discontinuities, one at 14 degrees C and one at 21 degrees C. The lower temperature discontinuity was associated...

Superoxide anion can modulate vascular smooth muscle tone and is potentially involved in diabetic vascular complications. The present study was undertaken to characterize both vascular production and the enzymatic source of superoxide anion in type 2 diabetic rats. In the thoracic aorta of OLETF rats, endothelium-dependent relaxation was markedly attenuated compared with that of control (LETO) ...

A mitochondrial defect was investigated in an infant with fatal congenital lactic acidosis (3-14 mM), high lactate-to-pyruvate ratio, hypotonia, and cardiomyopathy. His sister had died with a similar disorder. Resting oxygen consumption was 150% of controls. Pathological findings included increased numbers of skeletal muscle mitochondria (many with proliferated, concentric cristae), cardiomegal...

Although, starting with Straub’s diaphorase (4), a number of enzymes capable of oxidizing reduced nicotinamide adenine dinucleotide (NADH) have been isolated from respiratory chain preparations during the past 25 years (5-9), it has not been possible to identify even tentatively any of these as a relatively unmodified, soluble form of the primary dehydrogenase of the mitochondrial NADH oxidase ...

2,5-Furandicarboxylic acid (FDCA) is an important monomer for manufacturing biobased plastics. Biocatalysis has been recognized as a sustainable tool in organic synthesis. To date, the efficiencies of most biocatalytic processes toward FDCA remain low. So, it highly desired to develop efficient processes. In this work, route was developed by integrating cell-free extract galactose oxidase varia...