Recent findings revealed that the commonly used (15)N2 tracer assay for the determination of dinitrogen (N2) fixation can underestimate the activity of aquatic N2-fixing organisms. Therefore, a modification to the method using pre-prepared (15-15)N2-enriched water was proposed. Here, we present a rigorous assessment and outline a simple procedure for the preparation of (15-15)N2-enriched water....

We provide here a comparative genome analysis of 31 strains within the genus Paenibacillus including 11 new genomic sequences of N2-fixing strains. The heterogeneity of the 31 genomes (15 N2-fixing and 16 non-N2-fixing Paenibacillus strains) was reflected in the large size of the shell genome, which makes up approximately 65.2% of the genes in pan genome. Large numbers of transposable elements ...

Increases in global temperature are likely to have effects on the nitrogen cycle, including thosemediated through effects on legumes, which have a role in the N cycle by fixing N2. These effects may alter plant functioning and community structure, especially in N-limited ecosystems.Wemanipulated temperature and plant diversity in the field to investigate the effects of elevated temperature on a...

The circumboreal/circumpolar N2-fixing lichen Stereocaulon vesuvianum is among the most widespread and abundant fruticose species in montane Britain but has lost capacity to fix N2 over large areas of country. To investigate whether loss N2-fixation S. linked increased N deposition, we examined thallus morphology, physiology chemistry at twelve locations representing an deposition gradient 3–40...

N2 fixation should be a critical process in the nitrogen-poor surface water of the eastern Mediterranean Sea. Despite favorable conditions, diazotroph abundance and N2 fixation rates remains low for reasons yet explained. The main goal of this study was to investigate the limiting nutrients for diazotrophy in this oligotrophic environment. Hence, we conducted dedicated bottle-microcosms with ea...

Nodules from leguminous plants will continue to fix N2 into acid extractable compounds for several hours after they have been excised from the roots. The capacity of nodules to fix N2 is decreased twoto five-fold by slicing and is abolished by grinding the nodules (2). The decrease in nitrogen fixation which accompanies slicing of the nodules and suspending them in buffer or nutrient solutions ...

Nodules from leguminous plants will continue to fix N2 into acid extractable compounds for several hours after they have been excised from the roots. The capacity of nodules to fix N2 is decreased twoto five-fold by slicing and is abolished by grinding the nodules (2). The decrease in nitrogen fixation which accompanies slicing of the nodules and suspending them in buffer or nutrient solutions ...

mprovement of N2 fixation might be an effective strategy in peanut breeding for high yield under drought stress conditions. However, under water limited conditions peanut varieties having high water-use efficiency (WUE) are favorable. A pot experiment was conducted under greenhouse conditions at Khon Kaen University, Thailand during December 2002 to May 2003, and repeated during June 2003 t...

Dinitrogen (N2) fixation was investigated together with organic matter composition in the mesopelagic zone of the Bismarck (Transect 1) and Solomon (Transect 2) Seas (Southwest Pacific). Transparent exopolymer particles (TEP) and the presence of compounds sharing molecular formulae with saturated fatty acids and sugars, as well as dissolved organic matter (DOM) compounds containing nitrogen (N)...

Methods have been developed for identifying the pathway of assimilation of N2-derived nitrogen. The products of fixation of 13N-labeled nitrogen gas ([13N]N2), and the distribution of 13N within glutamine, were determined after short periods of labeling (approximately 1 to 120 s) and also in pulse-chase experiments. Ammonia, the amide nitrogen of glutamine, and the alpha-amino nitrogen of gluta...