Sodium channels in cultured neural cells were localized by light microscopic autoradiography of specifically bound 125I-scorpion toxin. Ninety percent of the cell-bound 125I-scorpion toxin was associated specifically with sodium channels as assessed by the blocking of autoradiographic labeling by unlabeled scorpion toxin and by depolarization. Sodium channels were distributed uniformly in the s...

The effects of TsIV-5, a toxin isolated from the Brazilian scorpion Tityus serrulatus, on whole-cell and single-channel Na currents were determined in N18 neuroblastoma cells. In whole-cell records at a test potential of -10 mV, external application of 500 nM TsIV-5 slowed inactivation 20-fold and increased peak current by about one-third without changing time-to-peak. Both the steady-state act...

Neurite outgrowth of C 1300 neuroblastoma cells, which were dispersed from adherent cultures or grown in suspension, was studied on different protein-coated surfaces. Of 29 different surface structures studied, including surfaces treated with various fibronectins, lectins, glycosidases, or glycosyltransferases capable of stimulating fibroblast spreading, only the surfaces coated with plasma fib...

The Brunn–Minkowski Theorem asserts that μd(A+B)1/d ≥ μd(A) + μd(B) 1/d for convex bodies A,B ⊆ R , where μd denotes the d-dimensional Lebesgue measure. It is well known that equality holds if and only if A and B are homothetic, but few characterizations of equality in other related bounds are known. Let H be a hyperplane. Bonnesen later strengthened this bound by showing μd(A+B)≥ (M1/(d−1) +N1...

Sodium currents mediated by voltage-sensitive sodium channels in normal and scorpion toxin-resistant neuroblastoma cells were measured using a giga-ohm seal recording method in the whole cell patch configuration. The voltage and time dependence of sodium currents were similar in normal and mutant cell lines. Half-maximal activation occurred for test depolarizations in the range of -7 to -11 mV....

Antibodies raised in rabbits and in guinea pigs against nerve growth factor-treated PC12 cells were absorbed exhaustively with three non-neuronal cell lines. In immunofluorescent staining experiments, these absorbed sera, designated anti-PC12, labeled specifically several different types of cultured neurons, including cerebral, cerebellar, spinal, dorsal root, and superior cervical neurons. Non...

We have developed idiotype-anti-idiotype monoclonal antibodies that provide evidence for rabies virus binding to the acetylcholine receptor (AChR). Hybridoma cell lines 7.12 and 7.25 resulted after fusion of NS-1 myeloma cells with spleen cells from a BALB/c mouse immunized with rabies virus strain CVS. Antibody 7.12 reacted with viral glycoprotein and neutralized virus infectivity in vivo. It ...

Turmeric, an essential ingredient of culinary preparations of Southeast Asia, contains a major polyphenolic compound, named curcumin or diferuloylmethane, which eliminates cancer cells derived from a variety of peripheral tissues. Although in vitro experiments have addressed its anti-tumor property, no in vivo studies have explored its anti-cancer activity in the brain. Oral delivery of this fo...