نتایج جستجو برای: n15
تعداد نتایج: 309 فیلتر نتایج به سال:
Flagellar class 3 operons of Escherichia coli and Salmonella are transcribed by RNA polymerase containing sigma 28. The consensus sequence of the sigma 28-dependent promoters was believed to be TAAA N15 GCCGATAA. In this study, we found that the E. coli genome contains a large number of sequences homologous to this consensus. However, we showed that they do not always exert a sigma 28-dependent...
In the Materials and Methods section, there is an error in the fifth and sixth sentences of the section titled “Flow cytometry.” This sentence should read: 2), PE-Cy5-conjugated anti-TCRβ (BioLegend, San Diego, CA), PE-Cy7-conjugated ant-NK1.1 (BioLegend), PE-Cy7-conjugated anti-CD8a (BioLegend), PE-CF594-conjugated anti-CD4 (BD Biosciences), and GPR30 (N15)-R Antibody (Santa Cruz Biotechnology...
The incorporation in vivo of compounds labeled with N15 or Cl4 into the purines and pyrimidines of nucleic acid has been repeatedly demonstrated (e.g. (l-3)). W e wish to report observations on the incorporation of radioactive erotic acid in vitro into the pyrimidines of the nucleic acid of rat liver slices. It is believed that this procedure will readily permit the study of precursors of nucle...
Recombineering technology in Escherichia coli enables targeting of linear donor DNA to circular recipient DNA using short shared homology sequences. In this work, we demonstrate that recombineering is also able to support recombination between a pair of linear DNA substrates (linear/linear recombineering) in vivo in E. coli. Linear DNA up to 100 kb is accurately modified and remains intact with...
Direct chemical evidence has shown that the infecting bacterial virus particle is extensively broken down during the reproductive process (1,2). This finding supports the interpretation of various studies of bacteriophage reproduction by geneticists, who have postulated that the virus is broken down to smaller units shortly after infection (3-6). The disruption of the virus particle upon infect...
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