A key cellular adaptation to diving in Weddell seals is enhanced myoglobin concentrations in their skeletal muscles, which serve to store oxygen to sustain a lipid-based aerobic metabolism. The aim of this study was to determine whether seal muscle cells are inherently adapted to possess the unique skeletal muscle adaptations to diving seen in the whole animal. We hypothesized that the seal ske...

Intracellular iron reportedly mediates many forms of tissue injury, including ischemic and myohemoglobinuric acute renal failure. This action may be explained by the ability of iron to catalyze the formation of the highly toxic hydroxyl radical (.OH) from H2O2 via the Fenton/Haber-Weiss reactions. To assess whether renal tubular myoglobin/iron loading, induced by a physiological mechanism (endo...

Myoglobin is an essential oxygen-binding hemoprotein in skeletal and cardiac muscles that buffers intracellular oxygen (O2) concentration in response to hypoxia or elevated muscle activities. We present a method that uses photoacoustic computed tomography to measure the distribution of myoglobin in tissue and the oxygen saturation of myoglobin (sO2-Mb ). From photoacoustic measurements of mice ...

Introduction Myoglobin is a monomeric hemoprotein that has captivated the interests of scientists and physicians for the past 60 years. It is a cytoplasmic hemoprotein that is expressed primarily within cells of striated muscle lineages of vertebrates (i.e. cardiomyocytes and oxidative skeletal muscle myofibers). Previous biophysical, cellular, molecular, pharmacological and physiological inves...

BACKGROUND Thrombolysis has become the standard therapeutic approach in patients with acute myocardial infarction. To identify patients who may benefit from early invasive procedures, reliable noninvasive assessment of success or failure of thrombolytic therapy is mandatory. METHODS AND RESULTS In a prospective study in 63 consecutive patients undergoing thrombolysis for their first myocardia...

Myoglobin is one of the most commonly used cardiac biomarkers for the clinical diagnosis of acute myocardial infarction, which is the leading cause of mortality worldwide. Herein, we report a novel ‘signal-on’ electrochemical immunosensing system for the quantitative detection of myoglobin without the need for natural enzymes and additional electron mediators. The assay was readily carried out ...

To measure myoglobin, a marker for myocardial infarction, directly in human serum, two-dimensional liquid chromatography in combination with electrospray ionization mass spectrometry was applied as an analytical method. High-abundant serum proteins were depleted by strong anion-exchange chromatography. The myoglobin fraction was digested and injected onto a 60 mm x 0.2 mm i.d. monolithic capill...

Biomedical applications adapt Nano technology-based transistors as a key component in the biosensors for diagnosing life threatening diseases like Covid-19, Acute myocardial infarction (AMI), etc. The proposed work introduces new biosensor, based on Graphene Field Effect Transistor (GFET), which is used diagnosis of Myoglobin (Mb) human blood. Graphene-based are faster, more precise, stronger, ...

Myoglobin is a cytoplasmic hemoprotein that is restricted to cardiomyocytes and oxidative skeletal myofibers and facilitates oxygen delivery during periods of high metabolic demand. Myoglobin content in skeletal muscle increases in response to hypoxic conditions. However, we previously reported that myoglobin-null mice are viable and fertile. In the present study, we define important functional...

Cytochrome b(5) (cyt b(5)), a component of endoplasmic reticulum membrane, plays a role in modulation of enzymatic activity of some cytochrome P450 (CYP) enzymes. The effect of apo-cytochrome b(5) on this enzymatic system has not been investigated in details, because preparation of cyt b(5) as a pure protein failed in many laboratories. In order to prepare the native apo-cytochrome b(5) in a la...