miliary tuberculosis (tb) is due to hematogenous spread of mycobacterium tuberculosis. clinical manifestations are nonspecific and protean, depending on the predominant site of involvement.we evaluated the clinical manifestations of 15 patients with miliary tb. the diagnosis of miliary tuberculosis was based on the identification of miliary nodules on chest radiography and one of the three foll...

the breast tuberculosis (tb) is an extremely rare manifestation for the reason that mammary cells offer great resistance to the survival and multiplication of mycobacterium infection. the diagnosis is difficult because of nonspecific clinical and radiological findings. it is usually suspected in a multiparous, reproductive woman. we report three cases of tubercular infection of breast confirmed...

  The identification of a large number of antigens with potential for development of new tuberculosis vaccine has been accomplished in recent years. This study was designed for cloning and expression of ESAT-6 as a potent antigen of Mycobacterium tuberculosis. Selected gene (Rv3875) was amplified by PCR and product was ligated into expressing plasmid vector pQE30 and recombinant pQE30-ES plasmi...

results results of the current study showed that the minimum inhibitory concentration value of cinnamaldehyde against m. tuberculosis was 200 µg/ml. moreover, rt-pcr data showed that a total of 25 genes were regulated by the cinnamaldehyde. of these, 12 genes were up-regulated, and 13 genes were down-regulated. conclusions cinnamaldehyde is a pattern to expand the new anti-tb drugs, because the...

A PCR test based on insertion sequence IS1081 was developed to detect Mycobacterium tuberculosis complex organisms in the peripheral blood. The method was applied to blood samples from immunocompetent individuals with localized pulmonary tuberculosis. Seven of 16 (43.75%) blood samples were found to be positive for the circulating DNA copies of M. tuberculosis complex.

Abstract Background Rapid identification of pathogenic Mycobacterium species is critical for a successful treatment. However, traditional method time-consuming and cannot discriminate isolated non-tuberculosis mycobacteria (NTM) at level. In the retrospective study, we evaluated clinical applicability PCR-reverse blot hybridization assay (PCR-REBA Myco-ID) with specimens rapid detection differe...

The polymerase chain reaction (PCR) using oligonucleotides based on the repetitive sequence (IS986) of Mycobacterium tuberculosis as a primer and the Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test (MTD), which combines an M. tuberculosis rRNA amplification method with the hybridization protection assay format, were evaluated for detection of M. tuberculosis in clinical samples. The ...

drug resistance (especially multiple drug resistance) in mycobacterium tuberculosis makes global concerns in treatment and control of tuberculosis. rapid diagnosis of drug resistant strains of the bacteria has vital importance in the prognosis of the disease. the aim of this study was to identify the mutations responsible for drug resistance in mycobacterium tuberculosis strains derived from pa...