Ribosomes from Mycobacterium tuberculosis (Mtb) possess species-specific ribosomal RNA (rRNA) expansion segments and ribosomal proteins (rProtein). Here, we present the near-atomic structures of the Mtb 50S ribosomal subunit and the complete Mtb 70S ribosome, solved by cryo-electron microscopy. Upon joining of the large and small ribosomal subunits, a 100-nt long expansion segment of the Mtb 23...

Tuberculosis remains today a major public health issue with total of 9 million new cases and 2 deaths annually. The lack an effective vaccine the increasing emergence strains Mycobacterium tuberculosis (Mtb) highly resistant to antibiotics, anticipate complicated scenario in near future. use nanoparticles features as alternative antibiotics tackling this problem due their potential effectivenes...

The Xpert MTB/RIF assay is a rapid and fully automated real-time PCR assay. The performance of the Xpert MTB/RIF assay as a primary screening test for urgent clinical specimens was evaluated during a 2-year period. The results showed that replacing smear microscopy with the Xpert MTB/RIF assay facilitates laboratory handling and improves the sensitivity and specificity of Mycobacterium tubercul...

An A1400G mutation of the rrs gene was identified in Mycobacterium tuberculosis (MTB) strain ATCC 35827 and in 13 MTB clinical isolates resistant to amikacin-kanamycin (MICs, >128 microg/ml). High-level cross-resistance may result from such a mutation since MTB has a single copy of the rrs gene. Another mechanism(s) may account for high-level amikacin-kanamycin resistance in two mutants and low...

A series of twelve novel hybrids cinnamic acid and thiocarbohydrazones were designed, synthesized in high yield using a simple coupling strategy via chlorides, evaluated for their impact against Mycobacterium tuberculosis (Mtb) cancer cells survival. Among them, compound 3 demonstrated strong anti-Mtb activity by reducing bacilli survival for>90 % all three treated Mtb isolates, whereas isoniaz...

Mycobacterium tuberculosis (Mtb) causes tuberculosis (TB). The treatment of TB requires administration of multiple drugs for long durations because of the unusual drug tolerance of Mtb. The phenotypic drug tolerance of genetically drug-susceptible Mtb in humans can be explained by its ability to form biofilms. Recent studies from different laboratories suggest that Mtb forms biofilms that harbo...

Tuberculosis (TB) is a serious infectious disease caused by a bacterial pathogen. Mortality from tuberculosis was estimated at 1.5 million deaths worldwide in 2013. Development of new TB drugs is needed to not only to shorten the medication period but also to treat multi-drug resistant and extensively drug-resistant TB. Mycobacterium tuberculosis (Mtb) grows slowly and only multiplies once or t...

Mycobacterium tuberculosis and atherosclerosis are major world health problems. In this report I will detail how to implement a java agent based model to simulate mycobacterium tuberculosis (Mtb) infection. This has been previously attempted successfully by professionals within this eld using C/C++. I will also discuss how to use the same approach for a di erent problem domain; atherosclerosis....

The sequencing of the Mycobacterium tuberculosis (MTB) H37Rv genome has facilitated deeper insights into the biology of MTB, yet the functions of many MTB proteins are unknown. We have used sensitive profile-based search procedures to assign functional and structural domains to infer functions of gene products encoded in MTB. These domain assignments have been made using a compendium of sequenc...

One-third of world population are infected with Mycobacterium tuberculosis (MTB) [1,2]. After exposure to MTB 30 % of the cases will develop latent infection, with a 5-10% lifetime risk of progression to active tuberculosis (TB) [2,3]. Extrapulmonary TB is observed in about 20% of the total cases. Although pulmonary and extrapulmonary tuberculosis cases have both decreased during the last years...