The clinical and public health importance of influenza and other respiratory viruses has accelerated the development of highly sensitive molecular diagnostics, but data are limited regarding preanalytical stages of diagnostic testing. We evaluated CyMol, an alcohol-based transport medium, for its ability to maintain specimen integrity for up to 21 days of storage at various temperatures; for it...

Reverse transcription (RT)-PCR assays have been widely described for use in the diagnosis of human parainfluenza viruses (HPIVs) and other respiratory virus pathogens. However, these assays are mostly monospecific, requiring separate amplifications for each HPIV type. In the present work, we describe multiplex RT-PCR assays that detect and differentiate HPIV serotypes 1, 2, and 3 in a combined ...

Three serotypes of foot-and-mouth disease virus (FMDV) including A, Asia1 and O, have been detected in Iran. Following the mass vaccination program which was implemented in all parts of the country in 2002, the number of outbreaks has been significantly reduced. Therefore, rapid detection of FMDV and its serotypes in clinical samples is essential for control of new outbreaks. In this study a re...

INTRODUCTION Enterovirus (EV) and parechovirus (PeV) can either infect humans asymptomatically or can cause gastroenteritis, respiratory symptoms and, sometimes, severe disease. As the number of newly identified EV and PeV genotypes keeps increasing, diagnostic methods need to be updated. To this end, we described a novel multiplex one-step real-time RT-PCR to detect EV and human PeV (HPeV) sim...

Virus dịch tả lợn Châu Phi (ASFV) và virus cổ điển (CSFV) đã gây thiệt hại kinh tế rất lớn cho người chăn nuôi lợn. Có nhiều phương pháp xét nghiệm để phát hiện hai này như: PCR, Realtime phân lập virus, ELISA. Bên cạnh đó Multiplex Real-time RT-PCR (mReal-time RT-PCR) đồng thời ASFV CSFV cũng được đánh giá ở một số nước trên thế giới nhưng chưa nghiên cứu tại Việt Nam. Trong chúng tôi mReal-ti...

Accurate and rapid diagnosis of Pandemic Influenza A/H1N1 2009 virus (H1N1 2009) infection is important for the prevention and control of influenza epidemics and the timely initiation of antiviral treatment. This study was conducted to evaluate the performance of several diagnostic tools for the detection of H1N1 2009. Flocked nasopharyngeal swabs were collected from 254 outpatients of suspecte...