potato tuberworm, phthorimaea operculella zeller (lepidoptera; gelechiidae) is a worldwide pest of solanaceous crops. larvae feed inside galleries in foliage, stems and tubers making chemical control unsuccessful, so other control methods should be applied. in recent years many plants have received genes that encode toxic proteins as a strategy to resist insect pests. in this study, optimal ph ...

A multi-objective optimization formulation that reflects the multi-substrate optimization in a multi-product fermentation is proposed in this work. This formulation includes the application of epsilon-constraint to generate the trade-off solution for the enhancement of one selective product in a multi-product fermentation, with simultaneous minimization of the other product within a threshold l...

Multi-electrophilic yet compact, the rigid α-methylene-β-lactone (MeLac) warhead creates new opportunities to probe live-cell proteome. The MeLac reacts with five types of nucleophile on all seven classes enzymes. Protein structures show reacting residues in red and blue. On upper left, a protein directs tethering an affinity ligand (yellow) probe. More information can be found full paper by X....

Enzymatic functions are often altered during disease onset and progression, and therefore chemical-biological studies, which utilize chemical knowledge to discover novel protein functions, are often employed to find proteins with functions closely related to disease phenotypes. Such studies are known as forward chemical-biological approaches and form part of the emerging field of enzymomics (om...

All members of the sulfotransferase (SOT, EC 2.8.2.-) protein family transfer a sulfuryl group from the donor 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to an appropriate hydroxyl group of several classes of substrates. The primary structure of these enzymes is characterized by a histidine residue in the active site, defined PAPS binding sites and a longer SOT domain. Proteins with this SOT d...

Laccases are multi-copper oxidases that oxidize a broad range of substrates at the expense of molecular oxygen, without any need for co-factor regeneration. These enzymes bear high potential for the sustainable synthesis of fine chemicals and the modification of (bio)polymers. Here we describe cloning and expression of five novel bacterial laccase-like multi copper oxidases (LMCOs) of diverse o...

The typical presentation of SARS-CoV-2 VIRUS infection is pulmonary involvement causing COVID 19 pneumonia. Although in recent times cases are being reported from different hospital that shows multi organ due to COVID-19 virus. Acute pancreatitis one the main presentations .here a case report not only acute but elevated liver enzymes non alcoholic disease along with gallbladder patient who was ...

Multienzyme cascade biocatalysis is an efficient synthetic process, avoiding the isolation/purification of intermediates and shifting reaction equilibrium to product side.. However, multienzyme systems are often limited by their incompatibility cross-reactivity. Herein, we report a multi-responsive emulsion proceed reactions sequentially for high reactivity. The achieved using CO2, pH, thermo-r...