Creatine kinase isoenzymes are separated electrophoretically on cellulose acetate, with use of an improved procedure for reactivation and visualization of enzymic activity. N-Acetyl cysteine is used as the reactivator and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide for visualization in a tetrazolium-coupled system. This test, both practical and reliable, is a suitable alter...

INTRODUCTION Honey is a common household product with many medicinal uses described in traditional medicine. Only recently has its antioxidant properties and preventive effects against disease been highlighted. Chrysin is a natural flavone commonly found in honey that has been shown to be an antioxidant agent. In this study, we investigated the antiproliferative and apoptotic effects of honey a...

Background & Aims: Plant extracts and plant-derived compounds are valuable sources of new medicinal agents that are commonly used to treat infectious diseases. The purpose of this study was to evaluate, antiLeishmanial activity of three plant extracts on L.major promastigotes by colorimetric assay as compared to a trivalent antimonial compound (tartar emetic). Methods: Leishmania major promasti...

1. Mesenchymal stem cell (MSC) therapy is drawing increasing attention in cardiology. However, the effect of aspirin, an assistant medication used extensively in the treatment of cardiovascular diseases, on MSC is not clear. 2. In the present study, we investigated the effect of aspirin on the growth of MSC in vitro and the underlying mechanism of its action. 3. The 3-(4,5-dimethyl-2 thiazoyl)-...

Objective: In this study, it was aimed to make in vitro characterization of the formulations by preparing hydrogel using chitosan, a biocompatible and natural polymer, PVP, synthetic polymer. addition, effects hydrogels containing FGF on proliferation keratinocyte cells were investigated. Methods: Within scope with different properties prepared their water absorption capacity, viscosities exami...

The knowledge about drug resistance in childhood leukemias and acute lymphoblastic leukemia (ALL) in general is limited. This is because of the lack of a suitable in vitro drug sensitivity assay, which is in part due to low in vitro ALL cell survival. We recently adapted the highly efficient 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay to test cells from ALL patient...

Objective: The role of norepinephrine (NE) on growth, adhesion and invasion methicillin-resistant Staphylococcus aureus (MRSA) ATTC 43300 was examined in human osteoblast (HOB) cells. effects NE and/or MRSA the viability cell death pathways HOB cells were also investigated. Furthermore, alterations bacterial response to oxidative stress (H2O2) analyzed presence/ absence NE. Materials Methods: B...

Abstract Despite the significant gains made in cancer therapy, remains a major cause of global deaths due to rapid drug resistance. Therefore, urgent concerted efforts towards discovery and development newer effective anticancer agents cannot be overemphasized. This study investigated vitro cytotoxicity potential methanol extracts root, stem, leaves Mezoneuron benthamianum . Leaf, stem root sam...

Breast cancer is the second most deadly diagnosed lifestyle disease among women. Surgery and chemotherapy are current treatments of choice; nevertheless, toxicity connected with this underscores urgency demand for human-friendly drug. 50% synthetic drugs available commercially today either direct or indirect descendants extracted from herbs. Anthocyanins possess many pharmacological activities,...

The lymphocyte stimulation test (LST) is one of the most useful laboratory tests for the identification of allergy to a specific drug. The present study was conducted to examine utility of the LST using the [3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide] (MTT) assay as a method for diagnosing drug allergy. The basic experimental conditions for the MTT assay were determined by u...