This study was carried out in order to determine the effects of boron and cold stress on pollen germination of almond hybrids in vitro culture, two different media including the absence and presence of boron (0 mg/l, and 100 mg/l) in 10% sucrose and 1% agar medium at 24°C in dark conditions. The results showed that different treatments had a significant effect on the germination percentage. The...

the effect of in planta tiba and proline on in vitro seedlings and cell culture of sugar beet was investigated. sterilized seeds were grown in vitro on ½ strength ms medium supplemented with 0 or 3 mg/l tiba. calli obtained on young leaves cultured on ms medium containing 1 mg/l bap, were used for the intiation of cell suspension cultures using ms basal composition supplemented with 0 or 50 m m...

Azotobacter vinelandii OP was grown to stationary phase in defined medium. The cell-free culture medium was analyzed for cytokinin content by XAD-2 and Sephadex LH-20 chromatography, thin-layer chromatography, tobacco callus bioassay, and enzyme immunoassay. Three cytokinin-active fractions were detected and tentatively identified as trans-zeatin, isopentenyladenosine, and isopentenyladenine. T...

Preimplantation genetic diagnosis (PGD) has successfully assisted couples with genetic diseases to conceive healthy babies during the past decades. However, biopsy of the blastomere has potential lesion to the embryos which commonly results in abortion. Thus, a noninvasive PGD is needed. In the past, the presence of genetic materials in maternal plasma or serum has triggered a great innovation ...

Background Follicle stimulating hormone (FSH) produced in recombinant Chinese hamster ovary (CHO) cells used for infertility treatment. Fetal Bovine Serum (FBS) in known as a factor in medium culture that provides the tolerance towards physiochemical variations and protects cells against apoptosis; on the other hand, FBS is the most expensive component in medium culture and collection that caus...

In all biological systems growth is defined as increase of chemical compounds. Bacteria can achieve to balanced growth if they are growing in a medium, which are completely adapted to it. Clostridium chauvoei, (Clostridium feseri) is an anaerobic, spore forming, motile, and polymorph bacteria, which its size varies from 0.5-1 to 3-8 micron and could be observed as individual bacterium, diplo, a...

BACKGROUND The question whether metacylic trypomastigote (MT) forms of different T. cruzi strains differentially release surface molecules, and how they affect host cell invasion, remains to be fully clarified. We addressed that question using T. cruzi strains that differ widely in the ability to invade cells. METHODOLOGY/PRINCIPAL FINDINGS Metacyclic forms were incubated at 37°C for 1 h in c...

Microbiology laboratories continually strive to streamline and improve their urine culture algorithms because of the high volumes of urine specimens they receive and the modest numbers of those specimens that are ultimately considered clinically significant. In the current study, we quantitatively measured the impact of the introduction of CHROMagar Orientation (CO) medium into routine use in t...

The aminoglycoside antibiotics, particularly gentamicin, are increasingly widely used and frequently in patients with impaired renal function. All these drugs are potentially ototoxic and measurement of blood levels is often desirable but may be complicated by the presence of a second drug, most commonly a penicillin or cephalosporin. Assays of streptomycin in the presence of penicillin have be...

Cultures of Tritrichomonas augusta, generously provided by Professor William Balamuth, were grown in Diamond's medium (6) modified by omission of agar. Because the organisms are anaerobic, the culture tubes were sealed with a mixture of vaseline and paraffin to exclude oxygen. 4-day cultures were fixed for 2 hr at 4°C in 5% glutaraldehyde-2% acrolein in 0.1 M cacodylate buffer with a final pH o...