The combination of SDS-PAGE and MS is one of the most powerful and perhaps most frequently used gel-based proteomics approaches in protein identification. However, one drawback of this method is that separation takes place under denaturing and reducing (R) conditions and as a consequence, all proteins with identical apparent molecular mass (Mr) will run together. Therefore, low-abundant protein...

The present study was designed to systematically investigate the chemical profile differences between crude Anemarrhenae rhizoma (CAR) and salt-processed Anemarrhenae rhizoma (SAR). Ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS), coupled with multivariate statistical analysis was used for the discrimination of chemical profiles and the i...

One of the main goals of proteomics research is the discovery of novel diagnostic markers and therapeutic targets. Currently, mass spectrometry is the main platform for analyzing complex protein samples. Lately, HPLC/MS-based approaches have gained considerable interest due to their larger potential for full automation when compared to gel-based techniques. Particularly, multi-dimensional HPLC-...

Wireless Local Area Network (WLAN) is susceptible to security provisioning in spite of the solutions such as the Wired Equivalent Protocol (WEP) or IEEE 802.1x. This paper proposes an integrated scheme for intrusion detection in WLAN systems. The proposed scheme operates with one or more Gathering Agents (GAs) and a Master Server (MS). Each GA is used to get security information by collecting t...

In this paper we present a feasibility of using a data-reductive strategy for analyzing big MS data. The proposed method utilizes our reduction algorithm MS-REDUCE and peptide deduction is accomplished using Tide with hiXcorr. Using this approach we were able to process 1 million spectra in under 3 hours. Our results showed that running peptide deduction with smaller amount of selected peaks ma...

Mass spectrometry (MS) is becoming increasingly popular in the field of structural biology for analyzing protein three-dimensional-structures and for mapping protein-protein interactions. In this review, the specific contributions of chemical crosslinking and native MS are outlined to reveal the structural features of proteins and protein assemblies. Both strategies are illustrated based on the...

Desorption/ionization on porous silicon mass spectrometry (DIOS-MS) is a novel method for generating and analyzing gas-phase ions that employs direct laser vaporization. The structure and physicochemical properties of the porous silicon surfaces are crucial to DIOS-MS performance and are controlled by the selection of silicon and the electrochemical etching conditions. Porous silicon generation...

Liquid chromatography with tandem mass spectrometry (LC-MS/MS) based proteomics is a well-established research field with major applications such as identification of disease biomarkers, drug discovery, drug design and development. In proteomics, protein identification and quantification is a fundamental task, which is done by first enzymatically digesting it into peptides, and then analyzing p...

Remarkable methodological advances in the past decade have expanded the application of liquid chromatography coupled with mass spectrometry (LC/MS) analysis of biotherapeutics. Currently, LC/MS represents a promising alternative or supplement to the traditional ligand binding assay (LBA) in the pharmacokinetic, pharmacodynamic, and toxicokinetic studies of protein drugs, owing to the rapid and ...