Aflatoxin B1 (AFB1) and ochratoxin A (OTA) are two of the most important mycotoxins associated with tropical subtropical climate as in Egypt. Therefore, this study aimed to isolated lactic acid bacteria (LAB) Bifidobacteria from some local dairy products Egypt using strains reduce/or remove AFB1 OTA toxins. Fifty samples, milk products, including ten samples each, Kareish, Damietta cheese (soft...

The isolation of non-O157 STEC from food samples has proved to be challenging. The selection of a suitable selective isolation agar remains problematic. The purpose of this study was to qualitatively and quantitatively evaluate six chromogenic agar media for the isolation of STEC: Tryptone Bile X-glucuronide agar (TBX), Rainbow® Agar O157 (RB), Rapid E. coli O157:H7 (RE), Modified MacConkey Aga...

Vibrio vulnificus was isolated from a bacteremic patient. This strain, together with other isolates of V. vulnificus, was compared with V. alginolyticus, V. fluvialis, and V. parahaemolyticus with regard to growth characteristics on enteric agar media (enabling isolation and identification) and production of exoenzymes which could correlate with invasive potential. V. vulnificus grew well on Ma...

The study was designed to determine the bacterial quality of local fried ground beef (Dambun nama) sold and consumed in Sokoto metropolis. Between January and December 2009, 216 samples of fried ground beef were collected and processed for bacterial isolations. Plate count agar, Violet Red bile lactose agar, RAPID E. coli agar and tellurite emulsion plates were used for the isolation of aerobic...

A comparison of several media, i.e., potato dextrose agar with olive oil (Oil-PDA), modified Dixon agar (mDIX) and variations of Leeming and Notman agar (LNA) for the isolation and growth of Malassezia and Candida species was examined. Since LNA supported the highest growth of Malassezia species its key components, i.e., ox bile, glycerol monostearate, glycerol and Tween 60, were added to CHROM...

A transposon Tn917 mutant of Listeria monocytogenes L028 was isolated on the basis of reduced growth on agar adjusted to pH 5.5. The disrupted gene, designated btlA (bile tolerance locus), encodes a putative secondary transporter of the major facilitator superfamily, which has significant homology to yxiO in Bacillus subtilis (lmo1417 in L. monocytogenes EGDe). The mutant demonstrated decreased...

Hart, Lewis T. (Louisiana State University, Baton Rouge), A. D. Larson, and C. S. McCleskey. Denitrification by Cornyebacterium nephridii. J. Bacteriol. 89:1104-1108. 1965.-Corynebacterium nephridii was found to reduce nitrate (contrary to the original description) at a rapid rate. In the conventional 0.1% nitrate broth, neither nitrite nor nitrate was detected after 24 hr. There was no assimil...

The purpose of this study was to evaluate the probiotic Bifidobacterium animalis subsp. lactis BB-12 with regard to its resistance to simulated gastrointestinal stress. The tested strain was able to survive both in the presence of 0.3% bile salts for 5h and in the acidified MRS broth with pH 2.0 for 2h, the rate of viability being by 78% and 76% respectively. The presence of pepsin with a view ...

A simple column filtration was developed to clarify meat homogenate suitable enough to quantify and assess the viability of bacterial population using flow cytometry. The removal of debris from homogenized meat suspensions was achieved by passing through different column materials such as sand particles, glass beads, dowex resin, and cell debris remover (CDR). Total plate counts on tryptic soy ...