C57BL/6 is a well-characterized mouse strain that is used extensively for immunological and neurological research. The establishment of C57BL/6 ES cell lines has facilitated the study of gene-altered mice in a pure genetic background-however, relatively few such lines exist. Using a defined media supplement, knockout serum replacement (KSR) with knockout DMEM (KSR-KDMEM), we find that we can re...

BACKGROUND The aim of this study was to evaluate fibroblast co-culture on in vitro maturation and fertilization of prepubertal mouse preantral follicles. MATERIALS AND METHODS The ovaries of 12-14 day old mice were dissected and 120-150 μm intact preantral follicles with one or two layers of granulosa cells, and round oocytes were cultured individually in α-minimal essential medium (α-MEM) su...

The Rockefeller University Press $30.00 J. Cell Biol. Vol. 207 No. 5 627–642 www.jcb.org/cgi/doi/10.1083/jcb.201405105 JCB 627 *B.K. Tripathi and X. Qian contributed equally to this paper. Correspondence to Douglas R. Lowy: [email protected]; or Brajendra K. Tripathi: [email protected] Abbreviations used in this paper: HBEC, human bronchial epithelial cell; IB, immunoblotting; IP, immunop...

Background: In mammalian ovaries, loss of over two-thirds of germ cells happens due to cell death. Nonetheless, the exact mechanism of cell death has yet to be determined. The present basic practical study was designed to detect 3 types of programmed cell death, namely apoptosis, autophagy, and necrosis, in murine embryonic gonadal ridges and neonatal ovaries.Methods: Twenty gonadal ridges and ...

The method to modify the surface of polystyrene (PS) substrate for stable and versatile biomolecule immobilization was developed, which consists of two steps: 1) surface treatment by argon plasma irradiation to introduce hydroxyl group on PS and 2) covalent immobilization of vinylmethyleter-maleic acid copolymer (VEMAC) through a coupling reaction between hydroxyl group on plasma-irradiated PS ...

Dermal fibroblasts and cutaneous nerves are important players in skin diseases, while their reciprocal roles during inflammation have not been characterized. Here we identified an inflammation-induced subset of papillary that promoted aberrant neurite outgrowth psoriasiform by secreting the extracellular matrix protein tenascin-C (TNC). Single-cell analysis fibroblast lineages revealed aTnc+pap...

Abbreviations: BRET, bioluminescence resonance energy transfer; EGFR, epidermal growth factor receptor; ER, endoplasmic reticulum; ERC, endocytic recycling compartment; FRET, fluorescence resonance energy transfer; GFP, green fluorescent protein; HEK, human embryonic kidney; IR, insulin receptor; MEF, mouse embryonic fibroblast; pNPP, p-nitrophenylphosphate; PDGFR, platelet derived growth facto...

BACKGROUND This study was conducted to evaluate fibroblast co-culture and Activin A on in vitro maturation and fertilization of mouse preantral follicles. METHODS The ovaries from 12-14-day-old mice were dissected, and 120-150 μm preantral follicles were cultured individually in α-MEM as based medium for 12 days. A total number of 456 follicles were cultured in four conditions: (i) base mediu...

Analysis of embryonic fibroblasts from GFP reporter mice indicates that the fibroblast cell type harbors a large collection of developmentally and phenotypically heterogeneous subtypes. Some of these cells exhibit multipotency, whereas others do not. Multiparameter flow cytometry analysis shows that a large number of distinct populations of fibroblast-like cells can be found in cultures initiat...